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Showing: 1501 - 1530 of 6111 results

  1. pst39-NST-clone14

    Plasmid
    #133421
    Purpose
    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
    Depositor
    Gerhard Wagner
    Insert
    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ORF for Nip1 encodes aa 4-156 (not complete ORF),…
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133421-plasmid-map-sequence-id-267716

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  13. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  14. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  22. pILGFP95

    Plasmid
    #83550
    Purpose
    Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Insert
    PDA1 promoter-yEGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PDA1
    Available since
    Dec. 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pDS24

    Plasmid
    #58952
    Purpose
    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93
    Insert
    Mitochondrially coded Arg8 protein
    Use
    Tags
    Expression
    Mutation
    Coding sequence synthetic in S. cerevisiae mitoch…
    Promoter
    n/a
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  27. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  28. pMJB1-RPS22B-Cl

    Plasmid
    #160431
    Purpose
    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter
    Depositor
    Gary Stormo
    Article
    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:
    Insert
    RPS22B (RPS22B Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Removed introns, shuffled codons to change nucleo…
    Promoter
    GAL1 and S. cerevisiae GAL1
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    160431-plasmid-map-sequence-id-317512

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Showing: 1501 - 1530 of 6111 results