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PD-1-miSFIT-Ce67

Plasmid

#124680

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

Plasmid

#204694

Purpose

This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 and NUT

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.2#2/Cre

Plasmid

#173661

Purpose

Expresses a NT.2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-4x

Plasmid

#124678

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

Plasmid

#222854

Purpose

Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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eGFP-PRM-5R

Plasmid

#112155

Purpose

Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-5R (ABL1 Human)

Expression

Mammalian

Mutation

contains only the PRM domain of Abl1

Promoter

CMV

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBV-Luc mut MBS1-4

Plasmid

#16565

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant MBS1-4

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-p300 (D1399Y)

Plasmid

#191763

Purpose

Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (D1399Y) (EP300 Human)

Tags

EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

Expression

Bacterial and Mammalian

Mutation

catalytic D1399Y mutation in p300

Promoter

EF1alpha

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B[1-560]

Plasmid

#213403

Purpose

Truncated human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B[1-560] (KIF5B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Article

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Tags

GFP derived from pMDC107

Expression

Plant

Promoter

35s core promoter

Available Since

Nov. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

Plasmid

#204696

Purpose

This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of the amino acids 355-505 of NUT

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

Plasmid

#204695

Purpose

This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(MIN) (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of majority of NUT, retaining only amino…

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVNP2.0-SpCas9

Plasmid

#206880

Purpose

Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

SpCas9 (cas9 )

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(FL)

Plasmid

#221243

Purpose

Expression of STING without transmembrane domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING without transmembrane domain (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-6-C, 16-C

Plasmid

#124681

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-TOP3B

Plasmid

#172423

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pD2sE_SC14

Plasmid

#219717

Purpose

DV2 stable E dimer

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC14

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

A259W, T262R, F279W, T280P

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSYC-176

Plasmid

#178961

Purpose

Expresses human GFAP fused to 3xFLAG tag in mammalian cells

Depositor

Seok-Yong Choi

Article

Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

Insert

Glial fibrillary acidic protein (GFAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94 Promoter

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-1x

Plasmid

#124679

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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