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Plasmid#126892PurposeGenome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
1167A
Plasmid#218233PurposeHDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae geneDepositorInsertputative metabolite transport protein
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAR-IF reporter
Plasmid#16628DepositorInsertCAR-IF
UseTagsbeta-galactosidaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAR-OF reporter
Plasmid#16627DepositorInsertCAR-OF
UseTagsbeta-galactosidaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp
Plasmid#185061PurposeEf1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445DepositorInsertU6:pegRNA:scaffold:PBS+RT template
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlueSKMimRNA
Plasmid#102535PurposeTemplate for synthesis of Minos transposase mRNADepositorInsertMinos transposase mRNA
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVG329
Plasmid#227771PurposeFor genomic replacement of YOR1 gene in budding yeastDepositorInsertGenomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)
UseTagsExpressionYeastMutationN/APromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG143
Plasmid#227754PurposeExpresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationLeu87IlePromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'SS
Plasmid#100991PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).DepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutantPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-COF1
Plasmid#31844DepositorInsertCofilin (COF1 Budding Yeast)
UseTagsGFP (S65T)ExpressionYeastMutationPromoterT7Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS316-GFP-COF1
Plasmid#31843DepositorInsertCofilin (COF1 Budding Yeast)
UseTagsGFP (S65T)ExpressionYeastMutationPromoterT7Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmRuby2-IRES-Clover
Plasmid#171059PurposeExpresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.DepositorInsertIRES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG134
Plasmid#227770PurposeExpresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationGlu84Gly mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG178
Plasmid#227761PurposeExpresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationGlu84KLys mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG144
Plasmid#227760PurposeExpresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationVal121Met mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG150
Plasmid#227759PurposeExpresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationSer137Asn mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG124
Plasmid#227758PurposeExpresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationThr83Ser mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG121
Plasmid#227757PurposeExpresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationGly80Glu mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG123
Plasmid#227755PurposeExpresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationAla157Val mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only