We narrowed to 62,126 results for: cloning

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pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBa.TfR.GFP

Plasmid

#45060

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

TfR (TFRC Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

LifeAct

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut4

Plasmid

#18087

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut4 (SLC2A4 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP20

Plasmid

#22573

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP20 (USP20 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR/pTER+ (430-1)

Plasmid

#17453

Purpose

Gateway entry vector for shRNA expression under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-Puro

Plasmid

#110841

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Promoter

Lac

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAVS1-tet-iCas9-BFP2

Plasmid

#125519

Purpose

all-in-one-inducible Cas9

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Insert

AAVS1-TET-ON 3G-SpCas9-P2A-BFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLVU/RED

Plasmid

#24178

Depositor

Lars Ittner

Article

Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

Insert

Chloramphenicol resistance gene (CmR) - ccdB gene

Use

Gateway Cloning and Lentiviral

Tags

DsRED

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pTetON-EGFP

Plasmid

#89453

Purpose

Tet/Dox-inducible expression of EGFP in mammalian cells (control)

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Article

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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