We narrowed to 2,116 results for: dcas9

1. pSB700-TTN-Puro

   Plasmid

   #128323

   Purpose

   SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)

   Depositor

   Alejandro Chavez

   Insert

   gRNA against human TTN for activation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEC578

   Plasmid

   #174366

   Purpose

   dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   dCas9 fused to SYNZIP18
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23150

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK340

   Plasmid

   #192639

   Purpose

   Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   SoxS has R93A and S101A mutations and dSpG has se…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCK341

   Plasmid

   #192640

   Purpose

   Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpRY
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   SoxS has R93A and S101A mutations and dSpRY has s…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK669

   Plasmid

   #192638

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dxCas9-NG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   SoxS has R93A and S101A mutations and dxCas9-NG h…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK412

   Plasmid

   #155371

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP416

   Plasmid

   #134996

   Purpose

   dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
   

   Use

   Synthetic Biology

   Tags

   3xFLAG, E coli Dam(N132A), and dCas9

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFD116

   Plasmid

   #124769

   Purpose

   pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   dcas9
   PpflB sgRNA BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ-NF1-L1-dSpRY

    Plasmid

    #177681

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ-NF1-L2-dSpRY

    Plasmid

    #177682

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB700-mouse-ACTC1-Puro

    Plasmid

    #128326

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSelect-6

    Plasmid

    #190243

    Purpose

    Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSelect-2

    Plasmid

    #190242

    Purpose

    Encodes lacI-targeting gRNA for positive selection of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSelect-7

    Plasmid

    #190244

    Purpose

    Encodes lacI- and pheA-targeting gRNAs for positive and negative selections of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSelect-9

    Plasmid

    #190245

    Purpose

    Encodes lacI- and aadA-targeting gRNAs for positive and negative selections of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge