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We narrowed to 2,062 results for: gem

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Showing: 1001 - 1020 of 2062 results

  1. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Publication
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

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  2. pKLV-EF1aGFP2AKAT7-linker-mAID-W

    Plasmid
    #159294
    Purpose
    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter
    Depositor
    Kosuke Yusa
    Publication
    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:
    Insert
    KAT7 (KAT7 Human)
    Use
    Lentiviral
    Tags
    mAID (auxin-inducible degron)-tagged
    Expression
    Mammalian
    Mutation
    CRISPR sites mutated (gRNA ID. 5 and A10)
    Promoter
    human EF1a promoter
    Available since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    159294-plasmid-map-sequence-id-313584

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  3. MultiMate-Cell-cycle

    Plasmid
    #206280
    Purpose
    Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.
    Depositor
    Imre Berger
    Publication
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
    Use
    Recombinant baculovirus production
    Expression
    Mammalian
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206280-plasmid-map-sequence-id-406629

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  4. pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

    Plasmid
    #242599
    Purpose
    A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.
    Depositor
    Joshua Wythe
    Publication
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)
    Expression
    Mammalian
    Promoter
    human EF1a
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242599-plasmid-map-sequence-id-495380

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  5. CmCitrine Lck w/ NosUTRs

    Plasmid
    #66785
    Purpose
    expression of fluorescent membranes in sea urchin primordial germ cells
    Depositor
    Amro Hamdoun
    Publication
    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10
    Insert
    Lck (LCK Human)
    Use
    Sea urchin, zebrafish, xenopus
    Tags
    3' UTR from sea urchin Nanos, 5' UTR fr…
    Mutation
    Tandem repeat of LCK the N-terminal membrane targ…
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pSBLego

    Plasmid
    #219967
    Purpose
    The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.
    Depositor
    Markus Seeger
    Publication
    Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10
    Type
    Empty backbone
    Tags
    SLEHHHHHH and pelB signal sequence
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    219967-plasmid-map-sequence-id-435554

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Showing: 1001 - 1020 of 2062 results