Addgene

We narrowed to 2,419 results for: gem

Showing: 1001 - 1020 of 2419 results

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pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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TRF2-HA

Plasmid

#200209

Purpose

Expression of full length mTRF2

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TRF2-HA (Terf2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-Polr2a promoter_600bps

Plasmid

#21111

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

Polr2a promoter_600bps (POLR2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pASH3 [pmyo-3::BeCNG1-YFP]

Plasmid

#168167

Purpose

Expression of BeCNG1-YFP in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

BeCNG1-YFP

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCas2F

Plasmid

#82377

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dN

Plasmid

#176465

Purpose

express VPS13D lacking N-terminal 117 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCas2D

Plasmid

#82375

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA

Plasmid

#225079

Purpose

Expresses precursor peptide ChrA in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

Plasmid

#225442

Purpose

BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

Depositor

Diego Orzaez

Insert

LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrH

Plasmid

#225084

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Use

Tags

His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrI

Plasmid

#225085

Purpose

Expresses anthrone-oxygenase like enzyme ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrI

Use

Tags

His6 and His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProC and no promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProD

Plasmid

#198431

Purpose

mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProD and no promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-CeSMG5_1-420-G91E_S

Plasmid

#147469

Purpose

Bacterial Expression of CeSMG5_1-420-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420-G91E (smg-5 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-BGG-PTC_E

Plasmid

#146191

Purpose

Mammalian Expression of BGG-PTC

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

BGG-PTC (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc-BGG-WT_E

Plasmid

#146192

Purpose

Mammalian Expression of BGG-WT

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

BGG-WT (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetMP-CeSmg7_1-395-G80E_S

Plasmid

#147476

Purpose

Bacterial Expression of CeSmg7_1-395-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395-G80E (smg-7 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 R19A

Plasmid

#121112

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R19A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 R20A

Plasmid

#121113

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R20A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R20A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 R22A

Plasmid

#121114

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R22A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R22A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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