We narrowed to 1,130 results for: ncl

1. pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

   Plasmid

   #146499

   Purpose

   Insect Expression of DmGW182

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmGW182 (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBetaActin-FKBP-Halo-Dync1h1motor

   Plasmid

   #191333

   Purpose

   Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBetaActin-FKBP-Dync1h1motor

   Plasmid

   #191332

   Purpose

   Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-Dync1h1motor (Dync1h1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-pSMANTIS_WT_1kb

   Plasmid

   #194187

   Purpose

   Includes the promoter (1kb) of SMTS (SMANTIS, MANTIS, AK125871)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SMANTIS promoter (1kb)

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

   Plasmid

   #190154

   Purpose

   Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp2 Rat)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only rat Vamp3 AA #1-81

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS97splitRBP_S2-Enz

   Plasmid

   #183768

   Purpose

   Expresses SHIP2-Enzyme domain flanked by RBP

   Depositor

   William Kerr

   Article

   Sudan et al Front Immunol. 2022 May 4;13:830961. doi

   Insert

   SHIP2 (INPPL1 Human)
   

   Use

   Tags

   6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

   Expression

   Bacterial

   Mutation

   includes aa K414 to aa V872 only

   Promoter

   T7lac

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmAGO2-RC_B

   Plasmid

   #145981

   Purpose

   Insect Expression of DmAGO2-RC

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   DmAGO2-RC (AGO2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A deletion of AA 51-56 and an insertion of 23 AA …

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. vPyACR_2164382

   Plasmid

   #153029

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_2164382
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_2164382 was C-terminally truncated to incl…

   Promoter

   CMV (+ enhancer)

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. vPyACR_21821

   Plasmid

   #153030

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_21821
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_21821 was C-terminally truncated to includ…

   Promoter

   CMV (+ enhancer)

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Puro-NCOA7g3 (BB24)

    Plasmid

    #139459

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAN19-TIR1-GUS-NOSt

    Plasmid

    #108547

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAN19-ccvTIR1-GUS-NOSt

    Plasmid

    #108548

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Changed F79 to G

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-BiFC-Venus

    Plasmid

    #105804

    Purpose

    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    VN173 (I152L); VC155

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid

    #71390

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MLRV

    Plasmid

    #118069

    Purpose

    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Inserts

    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHSN501

    Plasmid

    #50589

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos3.2FPP

    Plasmid

    #203758

    Purpose

    Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    HRas c-term (HRAS Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE-membrane-mEGFP

    Plasmid

    #105526

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    fragment encoding the plasma membrane targeting s…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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