We narrowed to 2,630 results for: organization Addgene

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pENTR_spatzle-HA

Plasmid

#240227

Purpose

Gateway entry clone with spatzle tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

spatzle (spz Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-hL1-5'_3.3kb plasmid

Plasmid

#226006

Purpose

Plasmid used to generate DNA-FISH probe targeting at human L1HS

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

Human L1 element 3.3kb sequences at the 5' end

Use

Synthetic Biology

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA1-EGFP

Plasmid

#226004

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA2-EGFP

Plasmid

#226005

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC

Plasmid

#200555

Purpose

Mammalian expression of secreted mouse CES2C

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC-S230A

Plasmid

#200557

Purpose

Mammalian expression of secreted mouse CES2C S230A mutant

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_47-300_mCh-SspB (pBS1071)

Plasmid

#185294

Purpose

For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I_47-300

Expression

Mammalian

Mutation

D125I

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

Plasmid

#185300

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC_98-130

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

Plasmid

#185296

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA_1-208

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_47-300_mCh-SspB (pBS1070)

Plasmid

#185293

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-SspB (pBS1068)

Plasmid

#185291

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Keratin-17

Plasmid

#57792

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-EB3-7

Plasmid

#57788

Purpose

Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

EB3 (MAPRE3 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

Plasmid

#235087

Purpose

GFP splicing reporter with FRG1 intron

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

Plasmid

#235086

Purpose

GFP splicing reporter with CORO1B intron

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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AICSDP-8:TOMM20-mEGFP

Plasmid

#87423

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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0_T7-mScarlet3

Plasmid

#225927

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AICSDP-7:SEC61B-mEGFP

Plasmid

#87426

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

SEC61B Homology Arms with mEGFP-linker (SEC61B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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