We narrowed to 1,322 results for: pET28a

1. USP7 G392D catalytic

   Plasmid

   #227203

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   G392D; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L469R FL USP7

   Plasmid

   #227225

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   L469R

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. E345K USP7 catalytic

   Plasmid

   #227211

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   E345K; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L373F USP7 catalytic

   Plasmid

   #227207

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   L373F; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. G392A USP7 catalytic

   Plasmid

   #227204

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   G392A; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. E345K FL USP7

   Plasmid

   #227224

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   E345K

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. V485G FL USP7

   Plasmid

   #227222

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   V485G

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. POC1481

   Plasmid

   #213807

   Purpose

   LguI-associated switch methylase

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Insert

   LguI-associated switch methylase M.XmnI
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. POC1482

   Plasmid

   #213808

   Purpose

   LguI-associated switch methylase

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Insert

   LguI-associated switch methylase M.XmnI
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_mouse-Caspase-1-H340D_enzymatic-domain

    Plasmid

    #183388

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    H340D

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest-894 (attR4-attR2)

    Plasmid

    #161893

    Purpose

    Multisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coli

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 2QO2 (EPHA3)

    Plasmid

    #101271

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pJK593

    Plasmid

    #71587

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK326

    Plasmid

    #71557

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes alanine-368 to aspartate

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK325

    Plasmid

    #71556

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes alanine-367 to aspartate

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK345

    Plasmid

    #71559

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletes alanine-367 and alanine-368

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK344

    Plasmid

    #71558

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletes alanine-367 (same as deleting alanine-368)

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK619

    Plasmid

    #72536

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ARL5:H14-K177 (2H16)

    Plasmid

    #25526

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL5:H14-K177 (ARL5A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids H14-K177

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. C064

    Plasmid

    #174353

    Purpose

    Plasmid for expressing frog PABPC1 (C-His) in E.coli

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (pabpc1.S Frog)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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