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Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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2QO2 (EPHA3)
Plasmid#101271PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPHA3 (EPHA3 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK593
Plasmid#71587PurposeProduces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE )
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK326
Plasmid#71557PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationchanges alanine-368 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK325
Plasmid#71556PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationchanges alanine-367 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK345
Plasmid#71559PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 and alanine-368PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK344
Plasmid#71558PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 (same as deleting alanine-368)PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK619
Plasmid#72536PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARL5:H14-K177 (2H16)
Plasmid#25526PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARL5:H14-K177 (ARL5A Human)
UseTagsHisExpressionBacterialMutationContains amino acids H14-K177PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET28_hTLN2-FL
Plasmid#164835PurposeExpression of FL human talin2DepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
UseTagsExpressionBacterialMutationsplit before mutationsPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4
Plasmid#26103PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-Expotin 1
Plasmid#42047DepositorInsertExportin-1 (XPO1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Ran(Q69L)
Plasmid#42048DepositorInsertRan (RAN Human)
UseTagsHisExpressionBacterialMutationQ69LPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-11 (AA100-373) (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET_scFv_QLuc
Plasmid#183261PurposeExpress scFv_QLuc in bacteria cellsDepositorInsertscFv_QLuc
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK438
Plasmid#71590PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only