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We narrowed to 9,096 results for: sgRNA

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Showing: 1001 - 1020 of 9096 results

  3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133344
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA_cpaA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133344-plasmid-map-sequence-id-266084

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  5. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid
    #102851
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3X Flag and NLS
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102851-plasmid-map-sequence-id-198995

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  7. Intron-Tagging-pX330-Cas9-mCherry

    Plasmid
    #159742
    Purpose
    Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.
    Depositor
    Stefan Kubicek
    Publication
    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    donor plasmid targeting sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159742-plasmid-map-sequence-id-314868

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  9. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Publication
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  10. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid
    #61069
    Purpose
    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)
    Depositor
    Filippo Del Bene
    Publication
    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    eGFPbait
    KalTA4
    Use
    Zebrafish expression
    Tags
    E2A
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61069-plasmid-map-sequence-id-99003

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  16. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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Showing: 1001 - 1020 of 9096 results