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pCAFNF-PSDd1.2-GFP

Plasmid

#125581

Purpose

Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

PSDd1.2-GFP (Dlg4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(WT)-mCherry

Plasmid

#87550

Purpose

PI(WT): Photo-inhibitable/blue light sensitive Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(Q61L)-mCherry

Plasmid

#87553

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Q61L

Promoter

CMV

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RAC1-G12V-deltaCAAX

Plasmid

#176095

Purpose

Constitutively active, nuclear localized Rac1

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RAC1 (RAC1 Human)

Expression

Mammalian

Mutation

G12V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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PLK4 gRNA (BRDN0001146388)

Plasmid

#76234

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK4 (PLK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-NGN2_WT

Plasmid

#215610

Purpose

For integration of NGN2 WT T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 (NEUROG2 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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CDK3 gRNA (BRDN0001146549)

Plasmid

#76763

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK3 (CDK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK3 gRNA (BRDN0001146559)

Plasmid

#76764

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK3 (CDK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK3 gRNA (BRDN0001147261)

Plasmid

#76765

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK3 (CDK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 3SA

Plasmid

#121467

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Mutation

S176A, T180A, S184A

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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NADK2 gRNA (BRDN0001487085)

Plasmid

#78093

Purpose

3rd generation lentiviral gRNA plasmid targeting human NADK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pF708-pET28a-Hs-delta38-METTL20-NHis wt

Plasmid

#85109

Purpose

expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta38) METTL20 (METTL20 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pF713-pEGFP-N1-Hs-FL-METTL20 wt

Plasmid

#85114

Purpose

transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hGRM1-RFP

Plasmid

#22920

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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PI4K2A gRNA (BRDN0001147595)

Plasmid

#76822

Purpose

3rd generation lentiviral gRNA plasmid targeting human PI4K2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PI4K2A (PI4K2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKD1 gRNA (BRDN0001146537)

Plasmid

#77228

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD1 (PRKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PDPK1 gRNA (BRDN0001146405)

Plasmid

#77453

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDPK1 (PDPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-mA93-RFP

Plasmid

#22918

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)

Use

AAV

Expression

Mammalian

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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SIK2 gRNA (BRDN0001148975)

Plasmid

#77139

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK2 (SIK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCQ gRNA (BRDN0001144886)

Plasmid

#75553

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCQ (PRKCQ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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