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pLS410

Plasmid

#239123

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLS418

Plasmid

#239128

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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p366_DG

Plasmid

#234687

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Avi tag

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

Avi tag and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p371_DG

Plasmid

#234694

Purpose

Golden gate domesticated pET28b with Intein-Chitin binding domain

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

Intein-Chitin binding domain

Expression

Bacterial

Mutation

No

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEEK1

Plasmid

#83488

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Kukil et al Microb Cell Fact. 2022 Jan 10;21(1):8. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Promoter

Ptrc2O

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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DVA_p15A_AH

Plasmid

#217602

Purpose

Destination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB U6

Plasmid

#229956

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK191

Plasmid

#226658

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK103

Plasmid

#226803

Purpose

Integrates KanR into NS1, 500 bp homology, one homologous HIP1 site in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK104

Plasmid

#226804

Purpose

Integrates KanR into NS2, 500 bp homology, one homologous HIP1 site in right homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK105

Plasmid

#226805

Purpose

Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK74

Plasmid

#226657

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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DVK_pBR322_FG

Plasmid

#217595

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_AF

Plasmid

#217593

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGB010

Plasmid

#223678

Purpose

pEF-HA-NLS-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGH1327

Plasmid

#223679

Purpose

pEF-HA-PYL1-NLSminus-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSL187

Plasmid

#223680

Purpose

pEF-HA-3xNES-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DVA_pBR322_AH

Plasmid

#217606

Purpose

Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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DVK_p15A_FG

Plasmid

#217585

Purpose

Destination vector with kanamycin resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge