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pET28_Caspase-4_p20_C258A

Plasmid

#214307

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

C258A

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-37

Plasmid

#214346

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL37 (IL37 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36γ

Plasmid

#214345

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36G (IL36G Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-SNAPf-mTurquoise2

Plasmid

#197494

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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TOM20-SNAPf-mTurquoise2

Plasmid

#197496

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the mitochondria.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCS4127

Plasmid

#131746

Purpose

Folinic acid switch Fol-UGAAG

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Folinic acid switch Fol-UGAAG

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-OCAM

Plasmid

#136767

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Ncam2

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Ncam2 (Ncam2 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-NQO1

Plasmid

#136766

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Nqo1

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Nqo1 (Nqo1 Mouse)

Use

In situ probe

Mutation

ISH Probe insert, V184A- Please see depositor co…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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JC021

Plasmid

#104944

Purpose

Estrogen inducing G-CSF

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

G-CSF

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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(PM-S608E)FLAG-Cep215-FL

Plasmid

#106906

Purpose

Expresses murine phospho-mimetic CEP215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Glutamic Acid

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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(PD-S608A)FLAG-Cep215-FL

Plasmid

#106905

Purpose

Expresses murine non-phosphorylatable Cep215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Alanine

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1_pIRES puro Glue

Plasmid

#73453

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-alpha1-chimaerin

Plasmid

#59317

Purpose

Expression plasmid for GFP-tagged alpha1-chimaerin (GFP at N-terminus of alpha1-chimaerin)

Depositor

Peter Scheiffele

Article

Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Insert

alpha1-chimaerin

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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tdEos-Paxillin-KM-C-10

Plasmid

#57654

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin KM

Tags

tdEos

Expression

Mammalian

Mutation

Mutation to remove Kozak sequence

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pB24

Plasmid

#61153

Purpose

Expression of Hnt1

Depositor

Charles Brenner

Article

Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

Insert

Hnt1 (HNT1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pSC-hTDG-K330A-PreE2

Plasmid

#52273

Purpose

Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

Ubc9-GST

Expression

Bacterial

Mutation

K330A, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pZS47a luxI-laa RBS

Plasmid

#48892

Purpose

ArsR-regulated expression of luxI-laa

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pArsR

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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