We narrowed to 92,138 results for: lias

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pZA3

Plasmid

#158481

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA2

Plasmid

#158480

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA11

Plasmid

#158489

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA9

Plasmid

#158487

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA4

Plasmid

#158482

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA10

Plasmid

#158488

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA12

Plasmid

#158490

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCIS-rPALcc

Plasmid

#145804

Purpose

expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Expression

Mammalian

Mutation

changed serine 767 to alanine to prevent glycosyl…

Promoter

CMV

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9FnCpf1GG

Plasmid

#131013

Purpose

Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

Plasmid

#127309

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127317

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSR358

Plasmid

#125112

Purpose

Expression of narL(1-131aa)-liaR(127-211aa) chimera under PLtetO-1, output Promoter PliaI121

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-liaR(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSR358.2

Plasmid

#125113

Purpose

Expression of narL(1-126aa)-liaR(122-211aa) chimera under PLtetO-1, output Promoter PliaI121

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-liaR(DBD)126
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127325

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

Plasmid

#127320

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

Plasmid

#127313

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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