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pJH1498
Plasmid#178600PurposePrgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-18 fused to GFPDepositorInsertINS-18 (ins-18 Nematode)
UseTagsExpressionWormMutationPromoterPrgef-1Available sinceJan. 5, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEM-Isce-Tol2-Tn5-neo
Plasmid#176619Purposefor generating PCR templates with Tol2 repeats and Isce sites for BAC recombinationDepositorInsertI-SceI sites, Tol2 repeats and Tn5-neo cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP821
Plasmid#164552PurposeExpresses Smo-FLAG K434RDepositorInsertSmoothened K434R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationOne of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP823
Plasmid#164554PurposeExpresses Smo-FLAG K448RDepositorInsertSmoothened K448R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationOne of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP822
Plasmid#164553PurposeExpresses Smo-FLAG K444RDepositorInsertSmoothened K444R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationOne of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr_2pabPAC
Plasmid#158971Purpose2P-activatable bPAC for lentivirusDepositorInsert2pabPAC
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW294
Plasmid#139491PurposeInsertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGRE2 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGRE2Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW295
Plasmid#139492PurposeInsertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpALD3 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpALD3Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP266
Plasmid#139488PurposeInsertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpCTT1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpCTT1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP264
Plasmid#139487PurposeInsertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP566
Plasmid#139500PurposeGene tagging vector to add mCherry Tag and membrane anchoring motifDepositorInsertmCherry-CaaX
UseTagsExpressionYeastMutationWTPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW200
Plasmid#139489PurposeInsertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGPD1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGPD1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW293
Plasmid#139490PurposeInsertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpHSP12 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpHSP12Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
UseTagsmCherryExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGC682
Plasmid#107020PurposeGFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motifDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS930
Plasmid#123054PurposeBeta-estradiol inducible DNA binding domainDepositorInsertZ3E [3a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC681
Plasmid#107019PurposeGFP reporter of lag-2 driven by 405 bp of promoterDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGC643
Plasmid#107016PurposeGFP reporter of lag-2 driven by 0.5 kb promoterDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKN3097
Plasmid#113392PurposeGalactose-inducible Ty3 with extra PPT- used for random bar code libraryDepositorInsertTy3
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProCDKA;1 GW
Plasmid#79750Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsnoneExpressionPlantMutationPromoterProCDKA;1Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA7
Plasmid#83871PurposeGateway donor vector for IAA7DepositorInsertIAA7 (IAA7 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA8
Plasmid#83873PurposeGateway donor vector for IAA8DepositorInsertIAA8 (IAA8 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA31
Plasmid#83864PurposeGateway donor vector for IAA31DepositorInsertIAA31 (IAA31 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA16
Plasmid#83854PurposeGateway donor vector for IAA16DepositorInsertIAA16 (IAA16 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW100ParsR
Plasmid#78635PurposepSB3K3 carrying J101-rbs32-arsR-t-ParsRDepositorInsertJ101-rbs32-arsR-t-ParsR
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW205J105-gfp
Plasmid#78644PurposepSB3K3 carrying J105-30gfp-tDepositorInsertJ105-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW203J116-gfp
Plasmid#78642PurposepSB3K3 carrying J116-30gfp-tDepositorInsertJ116-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW202J114-gfp
Plasmid#78641PurposepSB3K3 carrying J114-30gfp-tDepositorInsertJ114-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL1801
Plasmid#53039PurposePLlacO-1:RBS(st7):gfpDepositorInsertGFP
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlac0-1Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX mPtch-Cterm
Plasmid#58704Purposeused for anti-mouse Patched antibody production (for injection into animals)DepositorInsertPtch (Ptch1 Mouse)
UseTagsGSTExpressionBacterialMutationcontains only Cterminus (aa ~1238-1413)PromotertacAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 342
Plasmid#53012PurposePLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfpDepositorInsertsmCherry
araC
ompC
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -81 to +36 relative to start codonPromoterPlar, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1506
Plasmid#53026PurposePLlacO-1:RBS(st7):csrADepositorInsertcsrA
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pSS63
Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt10B Non-STOP
Plasmid#34748DepositorInsertWnt10B (Wnt10b Human)
UseGateway entry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt16 Non-STOP
Plasmid#35904DepositorInsertWnt16 (WNT16 Human)
UseEntry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt3 Non-STOP
Plasmid#35891DepositorInsertWnt3 (WNT3 Human)
UseEntry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt5B Non-STOP
Plasmid#35894DepositorInsertWnt5B (WNT5B Human)
UseEntry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt6 Non-STOP
Plasmid#35895DepositorInsertWnt6 (WNT6 Human)
UseEntry vectorTagsExpressionMutationSilent Mutation G (567) --> APromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt7B Non-STOP
Plasmid#35897DepositorInsertWnt7B (Wnt7b Human)
UseEntry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Wnt8B Non-STOP
Plasmid#35899DepositorInsertWnt8B (WNT8B Human)
UseEntry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only
