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pEF5-H4K16Q-Halo-FRT

Plasmid

#247458

Purpose

Expresses wild-type H4K16Q-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4K16R-Halo-FRT

Plasmid

#247457

Purpose

Expresses wild-type H4K16R-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-PAmCherry-FRT

Plasmid

#247337

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-HaloTag-FRT

Plasmid

#247338

Purpose

Expresses H2B-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4-HaloTag-FRT

Plasmid

#247452

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4K16A-Halo-FRT

Plasmid

#247459

Purpose

Expresses wild-type H4K16A-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

K16A

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW08K_0C5

Plasmid

#177280

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Pcat-cat

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA Q67H/N74D

Plasmid

#217442

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q67H,N74D

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61A)(HA)

Plasmid

#195025

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Mutation

T59/T61A

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61D)(HA)

Plasmid

#195026

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Mutation

T59/T61D

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (antisense)

Plasmid

#83817

Purpose

contains antisense mouse GATA3

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3-antisense strand (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pFCcGi

Plasmid

#59324

Purpose

Inducible expression of GFP and constitutive expression of mCherry

Depositor

Sophie Helaine, David Holden

Article

Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

Inserts

mCherry
EGFP

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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XE28 XBC 40

Plasmid

#16842

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pUC gRNA Shuttle

Plasmid

#47024

Purpose

Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Insert

gRNA Shuttle

Use

CRISPR; Cas9

Expression

Plant

Mutation

G to T cloning mutation at position 323

Promoter

Medicago truncatula U6.6

Available Since

Sept. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pTriEx-mCherry::LANS4

Plasmid

#60785

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS4

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-delta90GFP

Plasmid

#26645

Depositor

Anjen Chenn

Article

Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

Insert

delta90 beta-catenin (Ctnnb1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of first 90 aa of beta catenin

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-PsACR1

Plasmid

#85465

Purpose

Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

Insert

Synthetic construct PsChR1 gene

Tags

mCherry

Expression

Mammalian

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry::LANS1

Plasmid

#60784

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS1

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only