We narrowed to 2,388 results for: MEA

1. TMEM16F-T526K-peGFP-N1

   Plasmid

   #228580

   Purpose

   Expresses mouse TMEM16F-T526K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T526K plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS1862

   Plasmid

   #205975

   Purpose

   FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS1859

   Plasmid

   #205972

   Purpose

   FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS1860

   Plasmid

   #205973

   Purpose

   FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-E6AP Hect

   Plasmid

   #165102

   Purpose

   bacterial expression of a GST fusion of Human E6AP Hect domain

   Depositor

   Arthur Haas

   Article

   Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

   Insert

   E6AP Hect (UBE3A Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   E6AP Hect domain Amino acids 495-738 (5'QLNP…

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiPGK Puro DEST JNKKTR EE Clover

   Plasmid

   #90239

   Purpose

   Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (EE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS1801

   Plasmid

   #205966

   Purpose

   JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH proteins and CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS1232

   Plasmid

   #205969

   Purpose

   MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH proteins and CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1518

   Plasmid

   #205981

   Purpose

   FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHS1514

    Plasmid

    #205977

    Purpose

    FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8/Cse1 from MWEE01000013, Cas5-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHS1515

    Plasmid

    #205978

    Purpose

    FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas11/Cse2 from MWEE01000013, Cas5-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiPGK Puro DEST JNKKTR AE Clover

    Plasmid

    #90240

    Purpose

    Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (AE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFETCh_GABPA

    Plasmid

    #64253

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    GABPA Homology Arms (GABPA Human)
    

    Use

    Tags

    3X Flag P2A Neo

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160742

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFETCh_KMT2B

    Plasmid

    #101070

    Purpose

    Donor vector for 3' FLAG tag of human KMT2B

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KMT2B homology arms (KMT2B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFETCH_ATF1

    Plasmid

    #64689

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ATF1 Homology Arms (ATF1 Human)
    

    Use

    Tags

    3X Flag P2A Neo

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-p53 (1-73) (72P)

    Plasmid

    #62083

    Purpose

    Expression of human p53 (1-73) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72P

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. Venus-Bik-pEGFP-C1

    Plasmid

    #166737

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Bik (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTOPO-MCL1S159A

    Plasmid

    #21606

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 S159A (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 159 mutated to Alanine, abolishing phosph…

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pFETCh_FOXM1_iso1

    Plasmid

    #135734

    Purpose

    Donor vector for 3' FLAG tag of human FOXM1_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    FOXM1_iso1 Homology arms (FOXM1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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