Addgene

Showing: 1021 - 1040 of 1832 results

Results per page:

Sort results by:

pLV-shFMR1-mCherry

Plasmid

#222963

Purpose

Make lentivirus to knock down human FMR1 gene

Depositor

Xinyu Zhao

Article

Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

Insert

FMR1 shRNA (FMR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

Plasmid

#136688

Purpose

Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 (BECN1 Human)

Use

Tags

Trigger Factor

Expression

Bacterial

Mutation

DNA optimized for E. coli expression w/ three mut…

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 human PP2A Aα full length

Plasmid

#132634

Purpose

expresses human PP2A Aα full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A Aα (PPP2R1A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 human PP2A B56α full length

Plasmid

#132635

Purpose

expresses human PP2A B56α full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A B56α

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20 MEF2C

Plasmid

#89717

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-IRES-Tomato MEF2C

Plasmid

#89715

Purpose

Retroviral gene expression vector for MEF2C expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1-V16-hGDNF

Plasmid

#122035

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

hGDNF (GDNF Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTK Gatad2a flox

Plasmid

#110812

Purpose

Donor targeting vector for generating mouse Gatad2a conditional knockout allele

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20 MEF2C S222A

Plasmid

#89718

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed serine 222 to alanine

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBCX-Large-T-antigenK1 mutant

Plasmid

#189978

Purpose

Retroviral vector used to immortalize human cells

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

Large-T-antigenK1 mutant

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-IRES-Tomato MEF2C S222A

Plasmid

#89716

Purpose

Retroviral gene expression vector for MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-IRES_Tomato MEF2C S222D

Plasmid

#107231

Purpose

hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S222D

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLT3REVIR_MARK3 2573

Plasmid

#107233

Purpose

shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLT3REVIR_MARK3 1122

Plasmid

#107232

Purpose

shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-sgTrp53-sgAtrx-EFS-Cre

Plasmid

#189977

Purpose

Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

sgTrp53, sgAtrx, Cre recombinase

Use

Adenoviral, Cre/Lox, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-MerCreMer-hygro

Plasmid

#188982

Purpose

Retroviral expression of tamoxifen-inducible Cre recombinase

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

Tamoxifen-inducible Cre recombinase

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

Plasmid

#224006

Purpose

AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12754 - pAAV-AiE0680m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2754)

Plasmid

#224004

Purpose

AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge