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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-sgLkb1/Cre

Plasmid

#66894

Purpose

Expresses an Lkb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgLkb1

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_puromycin

Plasmid

#65309

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-APr

Plasmid

#61182

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pQF2wWB

Plasmid

#61313

Purpose

Enhancer trap version of QF2w, analogous to pGAWB

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF2w

Expression

Insect

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pQF2WB

Plasmid

#61312

Purpose

Enhancer trap version of QF2, analogous to pGAWB

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF2

Expression

Insect

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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U6>sgRNA(F+E)

Plasmid

#59986

Purpose

Empty vector for sgRNA cloning (F+E modified backbone)

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Type

Empty backbone

Use

CRISPR

Promoter

Ciinte.U6

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pT2SCb

Plasmid

#59385

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat2

Use

Template

Mutation

The 3’-end of the chloramphenicol resistance gene…

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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TRMPVneo.Yap.3093

Plasmid

#59915

Purpose

Tet on shRNA for conditional Yap1 knockdown

Depositor

Tyler Jacks

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

Yap1 (Yap1 Mouse)

Use

Retroviral

Available Since

Sept. 11, 2014

Availability

Academic Institutions and Nonprofits only

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Myc-Rab35 S22N

Plasmid

#47435

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Tags

Myc

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-tag

Plasmid

#55180

Purpose

FLAG-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

FLAG

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Actin-C-18

Plasmid

#53978

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Actin (ACTB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mKusabira Orange-C1 (mKO)

Plasmid

#54834

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

mKusabira Orange

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-LIN-41

Plasmid

#52716

Purpose

retroviral expression of human LIN-41 / TRIM71

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV31 L1L2

Plasmid

#52600

Purpose

Expresses HPV31 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV31 L1
HPV31 L2

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN

Plasmid

#50519

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
DS-ST1cas

Plasmid

#48647

Purpose

Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
ST1 tracrRNA

Use

CRISPR

Promoter

proC

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCoofy3

Plasmid

#43983

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCoofy4

Plasmid

#43986

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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