162,692 results

1. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

   Plasmid

   #182888

   Purpose

   Mammalian expression of PVALB mouse mAb HC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) heavy chain (PVALB Mouse)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pE5770

   Plasmid

   #170777

   Purpose

   expression of hybrid proteins in E. coli

   Depositor

   Anthony Schryvers

   Article

   Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

   Insert

   Kan resistance (kanR )
   

   Use

   Tags

   polyhisbioMbpTEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-sgRNA-MS2-U6

   Plasmid

   #171694

   Purpose

   To construct expression vector for tBE-V5-mA3

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   sgRNA scaffold-MS2-U6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-ccdB-boxB-tBE-V5-mA3

   Plasmid

   #171693

   Purpose

   Expresses tBE-V5-mA3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1a

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTC217

   Plasmid

   #70018

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREDCas9

   Plasmid

   #71541

   Purpose

   Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   lambda red genes
   plasmid curing system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCh-Rab7A

   Plasmid

   #61804

   Purpose

   Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5'UTR CGG 99x FMR1-EGFP

   Plasmid

   #63091

   Purpose

   Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZFN-AAVS1-KKR

    Plasmid

    #159298

    Purpose

    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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