We narrowed to 10,136 results for: Coli
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Plasmid#137036Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CGBE1 (pRZ3885)
Plasmid#140252PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorInserteUNG-BE4max(R33A)-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rs-FI-prk
Plasmid#162697PurposeExpresses R. spaeroides Form IC rubisco and S. elongatus PrkDepositorInsertR. spaeroides Form IC rubisco and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
TagsNLS from SV40 T antigen and mCherryExpressionMammalianPromoterCMV-tetAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFE-Selo-FI-prk
Plasmid#162698PurposeExpresses S. elongatus Form IB rubisco and S. elongatus PrkDepositorInsertS. elongatus Form IB rubisco and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Erythropoietin
Plasmid#182423PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusionDepositorInsertVNp-mNeongreen-eythropoietin (EPO Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rrub-FII-prk
Plasmid#162695PurposeExpresses R. rubrum Form II rubisco and S. elongatus PrkDepositorInsertsRubrum Form II rubisco
S. elongatus PRK
ExpressionBacterialPromoterPLtet0-1 promoter and under same promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLgw V5-EcoDam
Plasmid#59210PurposeMammalian DamID control lentiviral vector for expression of V5-Dam onlyDepositorInsertEcoDam
UseLentiviral; DamidTagsV5ExpressionMammalianPromoterHeat Shock Minimal Promoter and heat shock minima…Available SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Plasmid#85006PurposeFor Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.DepositorInsertEGFP-ires-tTA
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only