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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_SRC-1/1-IS2/2

Plasmid

#91251

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS2/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0319:Dendra

Plasmid

#159010

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0319

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0311:Dendra

Plasmid

#159052

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0311

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_1346:Dendra

Plasmid

#174997

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_1346

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM1-EGFP

Plasmid

#107794

Purpose

donor vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM1-eGFP (DNM1 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Expression

Bacterial and Yeast

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

Plasmid

#186967

Purpose

Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

DNMT3L (DNMT3L Human, Synthetic)

Use

Lentiviral

Tags

rTetR fusion

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmThor-V5His6

Plasmid

#79408

Purpose

Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor (Thor Fly)

Use

Schneider 2 cell expression

Tags

EGFP and V5-His6

Promoter

Ac5 promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCIneo-AR-S81E

Plasmid

#171224

Purpose

Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)

Depositor

Steven Balk

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

Insert

human androgen receptor Ser81Glu mutant (AR Human)

Expression

Bacterial and Mammalian

Mutation

AR-Ser81Glu (AR-S81E)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pECE-M2-BAIAP2 S366A

Plasmid

#31657

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 S366A (BAIAP2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 366 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
plko-tet-on-puromycin-shTRX1-259

Plasmid

#110920

Purpose

To knockdown Thioredoxin-1 following doxycycline addition

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Lentiviral

Promoter

unknown

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-2/5

Plasmid

#91385

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-2/5 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1-1/1-SV1/2

Plasmid

#91399

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV1/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-2/2

Plasmid

#91410

Purpose

Protein expression and purification of human SH3 domain construct GRB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-2/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP2-2/2

Plasmid

#91368

Purpose

Protein expression and purification of human SH3 domain construct GRAP2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP2-2/2 (GRAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8L2-1/1

Plasmid

#91526

Purpose

Protein expression and purification of human SH3 domain construct EPS8L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L2-1/1 (EPS8L2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pADSL-2XStrep

Plasmid

#125684

Purpose

expresses human ADSL protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ADSL (ADSL Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mqg*

Plasmid

#21175

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

Point mutation at nucleotide 6163 (numbering acco…

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
pCS2-mkgDUX4mal*

Plasmid

#21172

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

Point mutation at nucleotide 5114 (numbering acco…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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