We narrowed to 15,978 results for: grna

1. pWJ68

   Plasmid

   #46571

   Purpose

   Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   w-dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR APP shRNA 1

   Plasmid

   #30134

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   APP shRNA 1
   

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLentiCRISPRv2-sgMaob-HepmCherry

   Plasmid

   #192829

   Purpose

   Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 for sgRNA and hepatocyte-specific promoter (HS…

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC-crRNA-Cm

   Plasmid

   #158711

   Purpose

   Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   eforCP (for crRNA cloning)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   TetO

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A_dCas9-1x2

   Plasmid

   #63596

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1361Y

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SGL40C.EFS.dTomato

   Plasmid

   #89395

   Purpose

   Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLO1811_Cas9_noNLS_human

   Plasmid

   #74490

   Purpose

   Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSuper-gmiR-122

   Plasmid

   #171554

   Purpose

   a human miR-122-expressing plasmid

   Depositor

   Yan Zeng

   Article

   Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

   Insert

   miR-122 (MIR122 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB4070

    Plasmid

    #187811

    Purpose

    Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).

    Depositor

    Diego Orzaez

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    copper-inducible dCasEV2.1
    

    Expression

    Plant

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-LSL-mCherry-shLuci

    Plasmid

    #178590

    Purpose

    Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Luciferase
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ134-tRNA

    Plasmid

    #179213

    Purpose

    Golden Gate entry vector; 4th gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    no promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 RagB shRNA #1

    Plasmid

    #26627

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagB shRNA (RRAGB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRS267

    Plasmid

    #122380

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ133C

    Plasmid

    #69286

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ135A

    Plasmid

    #69277

    Purpose

    Golden Gate entry vector; 5th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ136A

    Plasmid

    #69278

    Purpose

    Golden Gate entry vector; 6th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRetroSuper-GFP Smad3

    Plasmid

    #15723

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    Smad3 shRNA (SMAD3 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEG302 22aa SunTag VP64 g4+g17

    Plasmid

    #120252

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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