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pNIC-HIS6-GST-TEV-AVITAG-GG

Plasmid

#215809

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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-24GFP

Plasmid

#25423

Depositor

David J. Goldhamer

Article

Yamamoto et al Mech Dev. . 124(9-10):715-28.

Insert

Myo D 5' flanking sequence (MYOD1 Human)

Use

Reporter construct

Tags

EGFP

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pREST-donor

Plasmid

#113807

Purpose

CRISPR donor plasmid to tag human transcription factor REST with GFP

Depositor

Kevin White

Insert

REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)

Use

CRISPR

Mutation

rs4864608

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pC-01

Plasmid

#166925

Purpose

sRNA part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

sRNA ToeHold - Block C- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pROSA26-ZtTA

Plasmid

#26082

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

a loxP-flanked β-geo preceding the tetracycline transactivator tTA

Use

Mouse Targeting

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEF003

Plasmid

#122838

Purpose

RBCS terminator with GreenGate C-D flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

RBCS terminator

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-HMGS

Plasmid

#108945

Purpose

heterologous expression of HMGS of E. faecalis in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA synthase (HMGS)

Tags

His6-Tag

Expression

Bacterial

Mutation

A110G

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCJ163

Plasmid

#175056

Purpose

rox flanked gentamycin resistance cassette integrates into A2542 integration site

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

rox-mR-rox

Use

Unspecified

Promoter

n/a

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLG371

Plasmid

#128410

Purpose

Cuminic acid-inducible system

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pspank(C)

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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