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pRS303-LacZ

Plasmid

#1169

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

LACZ

Tags

GAL1:10 promoter

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Publication

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Promoter

PfU6

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluu

Plasmid

#106122

Purpose

Fast indicator for synaptic glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Publication

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

synapsin

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52546

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Publication

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Expression

Bacterial

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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p6868 MSCV-IP N-V5 Zer1

Plasmid

#35100

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

Zer1 (ZER1 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

MSCV LTR

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6589 MSCV-IP N-HAonly CHEK2

Plasmid

#34903

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHEK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
PB_EF1a-DjCas13d-msfGFP-Blast

Plasmid

#226008

Purpose

DjCas13d protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Publication

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DjCas13d RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pbira(1-g78)-sec61b

Plasmid

#154455

Purpose

expresses flag-pbira(1-g78)-sec61b/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

flag-pbira(1-g78)-sec61b (SEC61B Human)

Expression

Mammalian

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Publication

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Publication

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Publication

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Promoter

U6

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD3

Plasmid

#114126

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pEMS1199

Plasmid

#29100

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ple53 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_BiPVe4_GCaMP6f

Plasmid

#213939

Purpose

AAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-S473A

Plasmid

#49190

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

Depositor

Peter Vogt

Publication

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

S473A

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
GAL 46Q+ProGFPp416

Plasmid

#15581

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Publication

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFP-CHAMP1

Plasmid

#159892

Purpose

Expresses CHAMP1 (CAMP) in mammalian cells

Depositor

Kozo Tanaka

Publication

Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CHAMP1 (CHAMP1 Human)

Tags

pEGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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