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GFP-GLT1b

Plasmid

#162516

Purpose

Rat GLT-1b cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSB116 - pL2_pSB90_fLUC-I_P19

Plasmid

#123191

Purpose

binary plant vector for transient TBSV P19 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::P19::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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HBT-GCaMP6-HA

Plasmid

#107433

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

GCaMP6S

Tags

HA

Expression

Bacterial

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pK1E-RNAP

Plasmid

#48144

Purpose

Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

rna polymerase K1E

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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M2e-noro-VLP

Plasmid

#201192

Purpose

Expresses noro-VLP decorated with influenza M2e in insect cells

Depositor

Vesa Hytönen

Article

Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

Insert

M2e-noro-VLP

Tags

Influenza M2e peptide

Expression

Insect

Promoter

Polyhedrin

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

Plasmid

#98975

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 4 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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SspB-HaloTag-DHPH-TIAM1

Plasmid

#176114

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107319

Purpose

Expresses SpCas9 fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15

Plasmid

#153523

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#15

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#15

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV P2Gm p53

Plasmid

#19751

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pmRFP-SUMO1 GG

Plasmid

#13990

Depositor

Jonathan Chernoff

Article

Dadke et al Nat Cell Biol. 2007 Jan . 9(1):80-85.

Insert

SUMO1 GG (Sumo1 Mouse)

Tags

mRFP

Expression

Mammalian

Mutation

C-terminal truncation. Cannot ligate to substrate…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pSB118 - pL2_pSB90_fLUC-I_HcPro

Plasmid

#123193

Purpose

binary plant vector for transient TEV HcPro and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHLP710

Plasmid

#203740

Purpose

Integration of OsTIR1-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHLP701

Plasmid

#203743

Purpose

C-terminal tagging of target proteins with 3xV5-IAA17 degron

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain

Use

Pcr amplification template for gene tagging

Tags

3xV5

Promoter

none

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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moPrP(3F4) D177N

Plasmid

#1319

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP D177N (Prnp Mouse)

Expression

Mammalian

Mutation

D177N point mutation

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

3F4 epitag

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

TET-On

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGA

Plasmid

#153530

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 1,991-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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