We narrowed to 20,611 results for: ATO

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pRK5_tAKARε

Plasmid

#119917

Purpose

Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARε

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pAKW.114A-PKA

Plasmid

#154051

Purpose

Expresses PKA in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)

Expression

Bacterial

Promoter

pCin

Availability

Academic Institutions and Nonprofits only

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pLN432 (FuGW-G14p-mKate2)

Plasmid

#105187

Purpose

Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G14p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS1

Plasmid

#120824

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS1 ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS2A

Plasmid

#120818

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS2A ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Long P2A

Plasmid

#164107

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRosetta_v2

Plasmid

#136477

Purpose

Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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M50 Super 8x TOPFlash

Plasmid

#12456

Purpose

Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pU6-hROSA26-1-1kbdonor

Plasmid

#234736

Purpose

PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

hROSA26-1 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMax_GFP

Plasmid

#177825

Purpose

Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-hSynapsin-Cre-WPRE

Plasmid

#86641

Purpose

Expresses Cre in mammalian cells

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

Cre

Use

Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

Expression

Mammalian

Promoter

hSynapsin

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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iCAP-ROOT

Plasmid

#192262

Purpose

AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

Depositor

Li Ye

Article

Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

Insert

Synthetic construct for ROOT VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1a

Plasmid

#205304

Purpose

Expression of WHaloCaMP1a in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-NMD3

Plasmid

#221296

Purpose

Expression of NMD3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NMD3 (pLxIS) (Nmd3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-AAVS1-5-1kbdonor

Plasmid

#234735

Purpose

PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

AAVS1-5 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCEC-red

Plasmid

#196040

Purpose

Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

Depositor

Paola Branduardi

Article

Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Grna cloning

Expression

Yeast

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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