We narrowed to 164,714 results for: DES

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pDEST-Myc-FBW7K404/412/R

Plasmid

#200716

Purpose

The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 K404 and K412 mutated to Alanines.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCav1 in pT3TS-Dest

Plasmid

#194293

Purpose

In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin (cav1.S Frog)

Use

In vitro transcription of mrna

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GLRX-roGFP2 in pT3TS-Dest

Plasmid

#194297

Purpose

In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

In vitro transcription of mrna

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

Plasmid

#194175

Purpose

Expresses lncRNA HIF1a-AS1 mutant

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Within Exon1, nucleotides 26-78 were removed

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEXP1-DEST-Noa1-HIS

Plasmid

#191972

Purpose

Destination vector containing HIS-tagged NOA1 coding sequence.

Depositor

Luca Scorrano

Insert

Noa1-HIS (NOA1 Human)

Use

Synthetic Biology

Tags

His

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-Noa1-GFP

Plasmid

#191968

Purpose

Gateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning Technology

Depositor

Luca Scorrano

Insert

Noa1-GFP

Use

Synthetic Biology

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::HA-RfA

Plasmid

#186404

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-V5-DEST-hSFRP1-GST

Plasmid

#189543

Purpose

Protein expression of SFRP1 in mammalian cells.

Depositor

Haian Fu

Insert

secreted frizzled related protein 1 (SFRP1 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::RfA

Plasmid

#186397

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA

Plasmid

#186396

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-Venus-RfA

Plasmid

#186371

Purpose

Destination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST tol2 UbB polyA

Plasmid

#188701

Purpose

Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA-HA

Plasmid

#186405

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PDEST-pSP172BSSPE-pFOGc::RfA-HA

Plasmid

#186406

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14_hsp-16.2p-grh-1-gfp

Plasmid

#177762

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Expression

Worm

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-dVHH[GFP] in pCSDEST

Plasmid

#162870

Purpose

Super-resolution of GFP in culture

Depositor

Rob Parton

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

mEos2-dVHH[EGFP]

Tags

mEos2

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-313 (attR4-attR3)

Plasmid

#161898

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3566 (pDEST15_Spt6 1223-1451)

Plasmid

#91814

Purpose

Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deleted residues 1-1222

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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