We narrowed to 19,373 results for: Kin

1. pCEP4-gp120(BaL)-CD4

   Plasmid

   #98957

   Purpose

   Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   HIV-1 (BaL) gp120
   

   Use

   Tags

   CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HSC1-D4Z4-GiP

   Plasmid

   #58542

   Purpose

   Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Inserts

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   D4Z4 repeat, partial
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1-alpha

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-SARS-CoV2-ORF8

   Plasmid

   #165089

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. FLAG-β-arrestin2 RRK/Q

   Plasmid

   #38261

   Depositor

   Robert Lefkowitz

   Article

   Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

   Insert

   β-arrestin2 (Arrb2 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Arg233, Arg237, and Lys251 converted to Glutamines

   Promoter

   CMV

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. FLAG-TPD52

   Plasmid

   #172452

   Purpose

   Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD52 (TPD52 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IPU-FLAG-NEK9T210A

   Plasmid

   #168270

   Purpose

   Expresses kinase-dead NEK9 mutant in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 210 to alanine

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. DJ-1 TEV site pET15b

   Plasmid

   #60687

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   TEV site was inserted (see comments below).

   Promoter

   T7

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pFAB3893

    Plasmid

    #47844

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 13 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-PS-Sos1 (PRM) - Cys (XSB364)

    Plasmid

    #78506

    Purpose

    Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Sos1 -Cys (PRM) (SOS1 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMALp2x-SCO5461(43-204)

    Plasmid

    #42509

    Depositor

    Koji Okamoto

    Article

    Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

    Insert

    guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
    

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-42 (nucleotide 1-126)

    Promoter

    tac

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGW1-Myc-DJ1-L166P/K130R

    Plasmid

    #29349

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Leu166Pro and Lys130Arg

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-4T1-Claspin-MΔN

    Plasmid

    #31624

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 899-1206 only

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pSG5-Myr-FLAG-p110α-DM

    Plasmid

    #55713

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    T207D, K227A

    Promoter

    T7

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1/GS-DJ1-L166P-V5

    Plasmid

    #29343

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    L166P

    Promoter

    Available since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pMIG-Flag-RIPK1-D324A

    Plasmid

    #102678

    Purpose

    retroviral expression plasmid for RIPK1-D324A

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and IRES-GFP

    Expression

    Mammalian

    Mutation

    CHANGE Glutamate 324 to Alanine

    Promoter

    unknown

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3578 pcDNA3 Bad S112A

    Plasmid

    #8797

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S112A

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only