We narrowed to 4,801 results for: pir

1. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. E17TAG GFP zeo resistance

   Plasmid

   #69118

   Purpose

   For E17TAG GFP reporter expression in non-zeocin resistant strains

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   E17TAG GFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17 to TAG

   Promoter

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Beta lactamase S68TAG

   Plasmid

   #68305

   Purpose

   Beta lactamase S68TAG gene

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   S68TAG beta lactamase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_LmFPPS

   Plasmid

   #186322

   Purpose

   pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   FPPS
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTcI39-PRM-wasabi

   Plasmid

   #195555

   Purpose

   Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

   Plasmid

   #202024

   Purpose

   Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpA T6A-L40A

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP01

   Plasmid

   #186260

   Purpose

   sfGFP under light light responsive PEL222 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1alpha-rtTA-Antares

   Plasmid

   #197587

   Purpose

   Expresses TET-On reverse transactivator labeled with Antares

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   rtTA-P2A-Antares
   

   Use

   Tags

   Antares

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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