We narrowed to 2,368 results for: pcas9

1. pYPQ158

   Plasmid

   #69307

   Purpose

   Gateway entry vector with hSpCas9 (Human codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9 (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1103

   Plasmid

   #129526

   Purpose

   pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH99

   Plasmid

   #128204

   Purpose

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH100

   Plasmid

   #128205

   Purpose

   improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL1315

   Plasmid

   #129527

   Purpose

   pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT02

   Plasmid

   #223374

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT04

   Plasmid

   #223376

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSK-Spsg

   Plasmid

   #214351

   Purpose

   Expresses cloning backbone for SpCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 tracrRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1211

   Plasmid

   #129521

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFlag-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH70

    Plasmid

    #128190

    Purpose

    Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPOR0SP0014

    Plasmid

    #117524

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-KA

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-KA (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K855A

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR0SP0009

    Plasmid

    #117522

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL90005

    Plasmid

    #117520

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-h (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Cas9-NRTH

    Plasmid

    #136925

    Purpose

    Expresses Cas9-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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