Skip to main content
Addgene
Showing: 1561 - 1590 of 5454 results

  4. pJK500

    Plasmid
    #72245
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and endogenous
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72245-plasmid-map-sequence-id-140434

    Enlarge

  5. pST50Trc4-HPCNDHFR

    Plasmid
    #63998
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63998-plasmid-map-sequence-id-177404

    Enlarge

  6. pST50Trc4-HPCDHFR

    Plasmid
    #63993
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63993-plasmid-map-sequence-id-177399

    Enlarge

  7. pST50Trc3-DHFRCYD

    Plasmid
    #63988
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63988-plasmid-map-sequence-id-177394

    Enlarge

  8. pST50Trc3-HPCDHFR

    Plasmid
    #63975
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63975-plasmid-map-sequence-id-177381

    Enlarge

  9. pST50Trc2-HPCNDHFR

    Plasmid
    #63962
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63962-plasmid-map-sequence-id-177368

    Enlarge

  10. pST50Trc3-FLAGDHFR

    Plasmid
    #63974
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63974-plasmid-map-sequence-id-177380

    Enlarge

  11. pST50Trc4-STRDHFR

    Plasmid
    #63991
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63991-plasmid-map-sequence-id-177397

    Enlarge

  12. pST50Trc3-HPCNDHFR

    Plasmid
    #63980
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63980-plasmid-map-sequence-id-177386

    Enlarge

  13. pST50Trc2-DHFRCYD

    Plasmid
    #63970
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63970-plasmid-map-sequence-id-177376

    Enlarge

  14. pST50Trc3-FLAGHISNDHFR

    Plasmid
    #63983
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63983-plasmid-map-sequence-id-177389

    Enlarge

  15. pST50Trc2-HPCDHFR

    Plasmid
    #63957
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63957-plasmid-map-sequence-id-177363

    Enlarge

  16. pCVD432

    Plasmid
    #63755
    Purpose
    Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.
    Depositor
    James Kaper
    Article
    Baudry et al J Infect Dis. 1990 Jun;161(6):1249-51.
    Insert
    EAggEC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pST50Trc1-HPCNDHFR

    Plasmid
    #63944
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63944-plasmid-map-sequence-id-177350

    Enlarge

  18. pST50Trc1-DHFRCYD

    Plasmid
    #63952
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63952-plasmid-map-sequence-id-177358

    Enlarge

  19. pST50Trc1-HPCDHFR

    Plasmid
    #63939
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63939-plasmid-map-sequence-id-177345

    Enlarge

  21. pKB234

    Plasmid
    #170021
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    hBD3
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170021-plasmid-map-sequence-id-335964

    Enlarge

  22. pEZYmyc-His

    Plasmid
    #18701
    Purpose
    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Tags
    Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18701-plasmid-map-sequence-id-181026

    Enlarge

Showing: 1561 - 1590 of 5454 results