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Plasmid#160430PurposeExpresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoterDepositorInsertRPL1B
UseTagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to vary nucleoti…PromoterS. cerevisiae GAL1Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K Δ2x+ve
Plasmid#112655PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K Δ2x+ve
UseTagsExpressionYeastMutationR59K, mutations (R223E, K224E, K225E, K233E, K234…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
UseTagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_NDT1
Plasmid#140593PurposeExpress NDT1 in S. cerevisiaeDepositorInsertNDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
UseTagsExpressionYeastMutationPromoterNDT1 promoter and 3'UTRAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFP88
Plasmid#83544PurposeUsed to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.DepositorInsertRPL15A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL15AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP77
Plasmid#83538PurposeUsed to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.DepositorInsertRPL8B promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL8BAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH3
Plasmid#83553PurposeUsed to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.DepositorInsertHXT7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterHXT7Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH0
Plasmid#83552PurposeUsed to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.DepositorInsertTPS1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTPS1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP90
Plasmid#83547PurposeUsed to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.DepositorInsertTEF2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF2Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP82
Plasmid#83541PurposeUsed to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.DepositorInsertYEF3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterYEF3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP80
Plasmid#83540PurposeUsed to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.DepositorInsertRPL3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP78
Plasmid#83539PurposeUsed to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.DepositorInsertSSB1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSB1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS1623
Plasmid#63795PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection markerDepositorInserttDimer2-Twin-Strep tag followed by HIS5
UseE. coli cloning vectorTagstDimer2-Twin-StrepExpressionMutationPromoternoneAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1322
Plasmid#63792PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection markerDepositorInserteCFP-Twin-tag followed by URA3
UseE. coli cloning vectorTagseCFP-Twin-tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1112
Plasmid#63784PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection markerDepositorInserteSapphire-Strep-tag II followed by URA3
UseE. coli cloning vectorTagsSapphire-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1522
Plasmid#63794PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3 selection markerDepositorInserteVenus-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseVenus-Twin-StrepExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1422
Plasmid#63793PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection markerDepositorInserteCitrine-Twin-tag followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Twin-tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1512
Plasmid#63788PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 selection markerDepositorInserteVenus-Strep-tag II followed by URA3
UseE. coli cloning vectorTagseVenus-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1312
Plasmid#63786PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection markerDepositorInserteCFP-Strep-tag II followed by URA3
UseE. coli cloning vectorTagseCFP-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E23K/F218A
Plasmid#19106DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE23K/F218APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pScSR beta deltaN Flag
Plasmid#13729DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHis and FlagExpressionBacterialMutationexpresses residues 31-244 of S. cerevisiae SRβ wi…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTSK561
Plasmid#190816PurposeFor C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiaeDepositorInsertHaloTag
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC-HYG-YR
Plasmid#61765PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).DepositorInsertsHygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1
Plasmid#87737PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_nat
Plasmid#184911PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only