We narrowed to 3,025 results for: ER

1. pFS321-pDestMyc-USP44catDead

   Plasmid

   #43902

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

   Insert

   USP44 (USP44 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C282A

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pTorPE-Y-GECO2s

   Plasmid

   #105067

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2s
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTorPE-Y-GECO2.1s

   Plasmid

   #105068

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2.1s
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFSW Syt1-C2B R398,399Q IRES GFP

   Plasmid

   #133819

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   R398,399Q

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSW Syt1-C2B K326,327E IRES GFP

   Plasmid

   #133820

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   K326,327E

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFSW Syt1-C2B D363,365N IRES GFP

   Plasmid

   #133822

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   D363,365N

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFSW Syt1-C2A D230,232N IRES GFP

   Plasmid

   #133824

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   D230,232N

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pF(UG) hSyn SYP-deltaTMD SYT7alpha

   Plasmid

   #179718

   Purpose

   Lentiviral expression of synaptic vesicle-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

   Plasmid

   #179722

   Purpose

   Lentiviral expression of lysosome-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pF(UG) U6-SYT7 sgRNA 777 HaloTag

    Plasmid

    #179724

    Purpose

    U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    sgRNA and HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pF(UG) CamKII sf iGluSnFR S72A

    Plasmid

    #179726

    Purpose

    Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    iGluSnFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S72A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Syt1 C2AB (96-421) D303G F234C

    Plasmid

    #170647

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX Syt1 C2AB (96-421) D303G V304C

    Plasmid

    #170648

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Syt1 C2AB (96-421) D365E F234C

    Plasmid

    #170650

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX Syt1 C2AB (96-421) D365E V304C

    Plasmid

    #170651

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX Syt1 C2AB (96-421) I367T F234C

    Plasmid

    #170653

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I367T, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX Syt1 C2AB (96-421) I367T V304C

    Plasmid

    #170654

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I367T, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. cfSGFP2-col4a1

    Plasmid

    #160317

    Purpose

    cfSGFP2-tagged collagen4a1 for mammalian expression

    Depositor

    Ikuo Wada

    Article

    Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

    Insert

    COL4A1 (COL4A1 Human)
    

    Use

    Tags

    cfSGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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