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pLz75_CB_operon_CsoS2_N3-N4

Plasmid

#140857

Purpose

Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-109 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLz76_CB_operon_CsoS2_N4

Plasmid

#140858

Purpose

Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids 1-189 of CsoS2

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz109_CsoS2_NTD

Plasmid

#140842

Purpose

His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 N-terminal domain (NTD)

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 262-869

Promoter

T7

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Use

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

trc

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2m

Plasmid

#105066

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPJ1

Plasmid

#83561

Purpose

Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER signal peptide

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS09

Plasmid

#52649

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS09

Use

Tags

Expression

Mammalian

Mutation

L414T mutated from GCaMP3

Promoter

T7

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS05

Plasmid

#52646

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS05

Use

Tags

Expression

Mammalian

Mutation

M373Q mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS08

Plasmid

#52648

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS08

Use

Tags

Expression

Mammalian

Mutation

T412Q/L414T mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
roGFP_iR_pQE30

Plasmid

#48634

Purpose

Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

roGFP1

Use

Tags

HisTag

Expression

Bacterial

Mutation

insertion R147B

Promoter

Available since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pFS320-USP44-catDead

Plasmid

#43901

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

C282A, T91A

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS322-USP44-catDeadMyc

Plasmid

#43904

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

HIS and MYC

Expression

Mammalian

Mutation

C282A, T91A

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS321-pDestMyc-USP44catDead

Plasmid

#43902

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

Expression

Mammalian

Mutation

C282A

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pTorPE-Y-GECO2s

Plasmid

#105067

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-Y-GECO2.1s

Plasmid

#105068

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.1s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSW Syt1-C2B R398,399Q IRES GFP

Plasmid

#133819

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

R398,399Q

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B K326,327E IRES GFP

Plasmid

#133820

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

K326,327E

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B D363,365N IRES GFP

Plasmid

#133822

Purpose

Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

D363,365N

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge