We narrowed to 22,348 results for: ESC;

1. pWPT-mEGFP

   Plasmid

   #190606

   Purpose

   Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral

   Mutation

   a deletion was created in mCherry CDS impairing i…

   Promoter

   EIF1-short

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GSX2-P2A-tGFP-DTA

   Plasmid

   #161750

   Purpose

   tGFP plasmid for the c-terminal targeting of human GSX2 locus

   Depositor

   Elena Cattaneo

   Article

   Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

   Insert

   turboGFP
   

   Use

   CRISPR

   Tags

   P2A-tGFP

   Expression

   Mammalian

   Promoter

   endogenous GSX2 promoter

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWKS8

   Plasmid

   #139415

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTFP1 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTFP1
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWKS17

   Plasmid

   #139420

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mCherry
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlg240

   Plasmid

   #101018

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   GluR 1 (Gria1 Rat)
   

   Expression

   Mammalian

   Mutation

   fluorescent protein inserted into subunit

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFlc045

   Plasmid

   #101017

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   GluR 1 (Gria1 Rat)
   

   Expression

   Mammalian

   Mutation

   fluorescent protein inserted into subunit

   Promoter

   cmv

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFlg034

   Plasmid

   #101015

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   GluR 1 (Gria1 Rat)
   

   Expression

   Mammalian

   Mutation

   fluorescent protein inserted into subunit

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB682

   Plasmid

   #60143

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW003-PpsbA-mNeonGreen

   Plasmid

   #205019

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   PpsbA

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCryptDel4.8

    Plasmid

    #141293

    Purpose

    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Inserts

    RelB
    gRNA targetting pMUT2
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Native promoter from pMUT2 relB/relE operon and P…

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid

    #113865

    Purpose

    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-Akt-FoxO3a-KTR-EGFP

    Plasmid

    #125131

    Purpose

    Fluorescent reporter for Akt activity

    Depositor

    Kazuhiro Aoki

    Article

    Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

    Insert

    FOXO2 (FOXO3 Human)
    

    Tags

    Enhanced green fluorescent protein (EGFP)

    Expression

    Mammalian

    Mutation

    FoxO3A 1-402 amino acid

    Promoter

    CAG promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTRW22K_7Ti1

    Plasmid

    #177293

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    lacZ
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Expression

    Bacterial

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Cre-T2A-mRuby2

    Plasmid

    #102989

    Purpose

    Expresses Cre and mRuby2

    Depositor

    Karin Scharffetter-Kochanek

    Article

    Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

    Inserts

    Cre (site-specific recombinase)
    mRuby2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJC49

    Plasmid

    #133060

    Purpose

    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mScarletI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-4511NES

    Plasmid

    #138375

    Purpose

    Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ERK
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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