We narrowed to 4,313 results for: Pet2

1. pET28sumo-hRig-I DCARDS

   Plasmid

   #210828

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

   Insert

   hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. BARD1 26-327 pET28a

   Plasmid

   #12646

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
   

   Expression

   Bacterial

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_mouse-pro-IL18

   Plasmid

   #183389

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (Il18 Mouse)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-LgBiT-SpyCatcher

   Plasmid

   #223634

   Purpose

   Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Tags

   His6, LgBiT

   Expression

   Bacterial

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-NLS-M-MLV-RT

   Plasmid

   #181804

   Purpose

   pET28a-NLS-M-MLV RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   NLS-M-MLV-RT
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b Apaf1 1-591

    Plasmid

    #26642

    Depositor

    Guy Salvesen

    Article

    Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

    Insert

    Apoptotic protease activating factor 1 (APAF1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acids 1-591

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Tags

    Polyhistidine

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b-Tipin-His

    Plasmid

    #23122

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

    Insert

    Tipin (TIPIN Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-pro-sumo1

    Plasmid

    #25101

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO1 (SUMO1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Changed Serine 2 to Alanine

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Tags

    6-His

    Expression

    Bacterial

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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