We narrowed to 23,954 results for: nes

1. pETM33_Nsp3c_SUD-M

   Plasmid

   #156470

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pU6-crRNA(TBK1)

   Plasmid

   #109322

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (TBK1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDonor-FMR1-CGG-BsmBI

   Plasmid

   #222966

   Purpose

   0CGG with BsmBI site for inserting CGG repeats

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   CGG repeats deleted from 5' UTR, BsmBI site …

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDonor-FMR1-dCGG0

   Plasmid

   #222965

   Purpose

   To create 0CGG FMR1 cell line

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1 homologous template no CGG repeats (FMR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   CGG repeats deleted from 5' UTR

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-Puro 2xStrep-HPC4-Gb5

   Plasmid

   #218177

   Purpose

   Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

   Depositor

   Barry Willardson

   Article

   Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

   Insert

   G beta subunit 5 (GNB5 Human)
   

   Use

   Lentiviral

   Tags

   2x Strep tag and HPC4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-syn-FLEX-tTA

   Plasmid

   #178516

   Purpose

   AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-syn-F14F15S-jGCaMP7s

   Plasmid

   #178514

   Purpose

   AAV vector for Flpo recombinase dependent jGCaMP7s

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   jGCaMP7s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-syn-mCre

   Plasmid

   #178515

   Purpose

   AAV vector for mCre recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mCre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4/TO-bio-myc-cCE

   Plasmid

   #112711

   Purpose

   For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   HIV Rev nuclear localization signal (NES), Stop c…

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV with Tet repressor binding sites

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-TTBG

    Plasmid

    #115232

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-TTBL

    Plasmid

    #115233

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-syn-Flpo

    Plasmid

    #174378

    Purpose

    also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Has Service

    AAV Retrograde

    Insert

    FLPo recombinase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-EF1a-HaloSFPQY527A

    Plasmid

    #166949

    Purpose

    Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Halo

    Expression

    Mutation

    SFPQ-Y527A

    Promoter

    eF1a

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UbC-Halo-SFPQY527A

    Plasmid

    #166947

    Purpose

    Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Halo

    Expression

    Mutation

    SFPQ-Y527A

    Promoter

    UbC

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KLC1N343S-Myc

    Plasmid

    #166963

    Purpose

    Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1-N343S (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    KLC1-N343S

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM33_Nsp3c_SUD-C

    Plasmid

    #156471

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-C (ORF1ab Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM33_Nsp3e_NAB

    Plasmid

    #156473

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139096

    Purpose

    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dxCas9 3.7
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DLG3_PDZ_2

    Plasmid

    #103947

    Purpose

    Protein expression and purification of NE-DLG PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-hACE2-hygro

    Plasmid

    #161758

    Purpose

    Expresses human ACE2 in mammalian cells

    Depositor

    Neville Sanjana

    Article

    Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge