We narrowed to 15,983 results for: grna

1. pTAG2E

   Plasmid

   #176865

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_2E
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-blast shGAC

   Plasmid

   #110410

   Purpose

   shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA Pol III)

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMKO.1 GFP B56 alpha shRNA 6-3

   Plasmid

   #10680

   Depositor

   William Hahn

   Article

   Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

   Insert

   PP2A B56 alpha shRNA (PPP2R5A Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. Blat-BSD

   Plasmid

   #163791

   Purpose

   The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TmiRc3_shNT

   Plasmid

   #81067

   Purpose

   Gateway entry vector encoding non-targeting shRNA

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   non-targeting shRNA
   

   Use

   Cloning

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroSuper-shYes

   Plasmid

   #26987

   Depositor

   Joan Massague

   Article

   Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

   Insert

   shRNA Yes (YES1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pSicoR-mCh-GFPi

   Plasmid

   #21905

   Depositor

   Miguel Ramalho-Santos

   Article

   Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

   Insert

   GFPi
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroSuper-GFP TIF1gamma

   Plasmid

   #15721

   Depositor

   Joan Massague

   Article

   He et al Cell. 2006 Jun 2. 125(5):929-41.

   Insert

   TIF1 gamma shRNA (TRIM33 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pR540

    Plasmid

    #207885

    Purpose

    template for 2 gRNA amplification

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    scaffold-pU6
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSuper-Rem2-2

    Plasmid

    #51595

    Purpose

    shRNA 2 against rodent Rem2

    Depositor

    Suzanne Paradis

    Article

    Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

    Insert

    Rem2 (Rem2, Rem2 Rat, Mouse)
    

    Use

    RNAi

    Promoter

    U6

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pLV(shRNA)-Puro-U6-CCEPR

    Plasmid

    #108277

    Purpose

    shRNA for CCEPR lncRNA

    Depositor

    Karl Munger

    Article

    Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

    Insert

    CCEPR (CCEPR Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-hU6-mU6-Cre2GO

    Plasmid

    #136907

    Purpose

    Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Banshee-Sh-Ctrl

    Plasmid

    #85587

    Purpose

    Control hairpin sequence used in gene knockdown experiements

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    sh-Ctrl
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAPM sh2 DNMT1

    Plasmid

    #88885

    Purpose

    Lentiviral vector expressing shRNA against murine DNMT1

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

    Insert

    shRNA no.2 DNMT1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV (spleen focus forming virus)

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFTK093

    Plasmid

    #171365

    Purpose

    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330(puro) Rosa26-H3F3B

    Plasmid

    #73131

    Purpose

    Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    spCas9
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mammalian_SaAID3S

    Plasmid

    #188651

    Purpose

    Expresses SaAID3S and gRNA in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    nSaCas9-AID3S--UGI
    Sa-sgRNA
    

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    EF-1a and H1

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mammalian_SaAID2S

    Plasmid

    #188652

    Purpose

    Expresses SaAID2S and gRNA in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S-nSaCas9--UGI
    Sa-sgRNA
    

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    EF-1a and H1

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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