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pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Expression

Mammalian

Mutation

S472A

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-hspB5

Plasmid

#63096

Purpose

Expression of HSPB5 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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DRH334: FCK-QuasAr2-mO2

Plasmid

#51692

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHes1(467)-luc

Plasmid

#41723

Depositor

Ryoichiro Kageyama, Raphael Kopan

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes1 Promoter (-467 to +46) (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter (-467 to +46)

Promoter

Hes1 promoter fragment (-467 to +46)

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF1-PTPRD-WT

Plasmid

#25642

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

An NsiI restriction site was engineered into the …

Available Since

April 21, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-MIB1-IRES-Puro

Plasmid

#140240

Purpose

Lentiviral vector expressing flag-tagged MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Flag-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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FU-mILa-CRW

Plasmid

#84556

Purpose

lentiviral expression of mouse IL1a and mRFP

Depositor

Li Xin

Article

Zhang et al Mol Cell. 2016 Sep 15;63(6):976-89. doi:

Insert

IL1a (Il1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

Plasmid

#87803

Purpose

Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JSAP1_WT

Plasmid

#208772

Purpose

Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JSAP1 (MAPK8IP3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

Plasmid

#161577

Purpose

Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-10nm-EBFP2

Plasmid

#231184

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

10nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EF.delmFasL.CMV.GFP

Plasmid

#17620

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Insert

delFasL (Fasl Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Noncleavable mouse delFasL . Deleted the exon 2 r…

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCLXSN-c-JUN

Plasmid

#102758

Purpose

Expression of c-Jun

Depositor

Jin Chen

Article

Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

Insert

c-Jun (JUN Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pIDTSmart-CMV-PLRS1

Plasmid

#217365

Purpose

Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

Depositor

Abhishek Chatterjee

Article

Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

Insert

leucyl-tRNA synthetase polyspecific mutant

Expression

Mammalian

Mutation

M40I, T252A, Y499I, Y527A, H529G

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCLXSN-EphA2-Flag

Plasmid

#102755

Purpose

Expression of EphA2

Depositor

Jin Chen

Article

Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

Insert

EphA2 (EPHA2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A_BAF_G47E

Plasmid

#101774

Purpose

Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

G47E mutation

Promoter

EF1a

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CD-MPR-mCherry-RUSH

Plasmid

#202798

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

Plasmid

#139087

Purpose

Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p2K7-bsd-UBI-tagRFP-KDEL

Plasmid

#114179

Purpose

Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Lentiviral

Tags

KDEL retention signal and signal peptide

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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