We narrowed to 14,197 results for: shi

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pCPH2A.X_K134A-GS3

Plasmid

#204752

Purpose

Expression of Cas9 and human H2AX with the K134A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134A mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT46

Plasmid

#218724

Purpose

Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT46 (IFT46 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT25

Plasmid

#218721

Purpose

Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT25 (HSPB11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC

Plasmid

#213535

Purpose

Expresses the split transcription factors in cultured neuron and mouse brain

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

Synapsin

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(HA)

Plasmid

#195023

Purpose

HA-tagged Creb5 in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128)

Plasmid

#195031

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PFKM_sgRNA1

Plasmid

#201610

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PFKM_sgRNA2

Plasmid

#201611

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK1-hygro

Plasmid

#199640

Purpose

sgRNA guide against VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-In18runon-PTPN22

Plasmid

#195377

Purpose

A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Use

Gateway donor vector

Mutation

Intron 18-runon PTPN22

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-In18runon-PTPN22

Plasmid

#195379

Purpose

Mammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Intron 18-runon PTPN22

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Ex1-17-PTPN22

Plasmid

#195375

Purpose

Mammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Tags

FLAG

Expression

Mammalian

Mutation

PTPN22 mutant containing exon 1 to 17

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_591

Plasmid

#160653

Purpose

Expresses OLE1 under the control of the yeast Sup35 promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

YGL055W (OLE1 Budding Yeast)

Expression

Yeast

Promoter

Sup35 promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Plasmid

#186703

Purpose

Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A, R280A,K284A]

Plasmid

#188599

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E1-#2

Plasmid

#171519

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-742pUC19-tNGFR-P2A-Caspase8

Plasmid

#186099

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CASPASE8

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmGamillus

Plasmid

#182971

Purpose

Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmGamillus

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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