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Plasmid#65985PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23102:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23102_FB
Plasmid#65986PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23102:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23102_GB
Plasmid#65987PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23102:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103_AB
Plasmid#65988PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23103:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103_EB
Plasmid#65989PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23103:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103_FB
Plasmid#65990PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23103:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23100_AB
Plasmid#65980PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK360
Plasmid#71571PurposeProduces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)DepositorInsertacetyl-CoA:oxalate CoA-transferase
UseTagsExpressionBacterialMutationchanges threonine-2 to alaninePromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK385
Plasmid#71572PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICSL11062
Plasmid#68256PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala1
Plasmid#65814PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterInsulated waaHp from E. coliAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-RFP SADBs
Plasmid#67153PurposepSG5 containing N-terminally RFP tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsRFPExpressionMammalianMutationPromoterSV40Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-HA SADBs
Plasmid#67149PurposepSG5 containing N terminally HA tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterSV40, T7Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-GST-C11orf83
Plasmid#65848Purposebacterial expression of GST (N-ter) - C11orf83/UQCC3DepositorInsertUQCC3 WT (UQCC3 Human)
UseTagsGSTExpressionBacterialMutationWTPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F65A
Plasmid#65092PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange phenylalanine 65 to alanine (KDR backbone)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1410
Plasmid#53011PurposepLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
ompC
rpoS
UseSynthetic BiologyTagsT7noRBS (900bp) and gfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83A
Plasmid#58420PurposeExpresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, and 83 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-D92N
Plasmid#58412PurposeExpresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationApartate 92 to AsparaginePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24,88,104A
Plasmid#58406PurposeExpresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysines 24, 88, and 104 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only