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pCRISPR_eGFP_369

Plasmid

#111823

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 349-369

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3BΔHECT-IRES-Puro

Plasmid

#107347

Purpose

Expresses HA tagged UBE3B deletion of HECT domain in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3BΔHECT (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

deletion HECT domain (757-end)

Promoter

CMV

Available Since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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p-mCherry-N1-iChloC_CA

Plasmid

#98171

Purpose

slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct iChloC_C128A gene

Tags

mCherry

Expression

Mammalian

Mutation

E83Q, E90R, E101S, C128A, T159C, D156N

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(150-599)

Plasmid

#90108

Purpose

Mammalian expression of Flag-MCPIP1(150-599)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iL

Plasmid

#83313

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iL

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

Promoter

T5

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
MAGE-A3Pro pGL4basic

Plasmid

#78138

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A3 (MAGEA3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -418/+12 of promoter

Promoter

MAGE-A3

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

cmv

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-tagBFP

Plasmid

#65266

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shGL2

Plasmid

#60004

Purpose

Co-expresses control GL2 shRNA and GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Expression

Mammalian

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + T356

Plasmid

#58911

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T356 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S780

Plasmid

#58915

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S780 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S788

Plasmid

#58916

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S788 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S807

Plasmid

#58918

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S807 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 shPIK3CD.v1 puro

Plasmid

#58706

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 shTGFBR3 puro

Plasmid

#58696

Purpose

Lentiviral shRNA vector for knockdown of human TGFBR3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shTGFBR3 (TGFBR3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-L3-oPRE-L2

Plasmid

#32415

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

oPRE

Use

Gateway Cloning

Mutation

WPRE with deletion of X protein promoter and muta…

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only

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EYFP-Abr

Plasmid

#36414

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Tags

EYFP

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only
pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only