We narrowed to 18,863 results for: REV;

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

Plasmid

#174876

Purpose

PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser2ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B WT

Plasmid

#123092

Purpose

Expresses 3xFLAG-LC3B wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAT9651-BEAR-GFP

Plasmid

#162989

Purpose

BEAR target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9752-BEAR-mScarlet

Plasmid

#162991

Purpose

BEAR target plasmid with split mScarlet and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSNR52-sgTEF1

Plasmid

#46922

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgTEF1 promoter (TEF1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFlp-Km

Plasmid

#222334

Purpose

Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-NES

Plasmid

#64727

Purpose

Cytosol-targeted, FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

Insert

AKAR4-NES

Tags

Cerulean, NES, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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AAV8R-12 Capsid

Plasmid

#213968

Purpose

AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

Depositor

Zhonghua Lu

Article

Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

Type

Empty backbone

Use

AAV

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ:Citrine-TurboID-3xMyc

Plasmid

#199244

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID-3xMyc

Tags

TurboID-3xMyc

Expression

Plant

Promoter

Arabidopsis UBQ10 promoter with intron

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1

Plasmid

#163625

Purpose

Expresses DCLK1

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Promoter

sv40

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2m

Plasmid

#176842

Purpose

Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir2-LNS2

Plasmid

#220086

Purpose

C-terminal PA binding domain of Nir2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM1 (PITPNM1 Human)

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 896-1216

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Ntn1-Fc-His

Plasmid

#72104

Purpose

Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn1 (Ntn1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas G12V

Plasmid

#18666

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

G12V mutation in HRas

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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