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pDONR223_MAP2K1_WT

Plasmid

#82139

Purpose

Gateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.T19N

Plasmid

#82182

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway entry vector

Mutation

T19N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKBKE_WT

Plasmid

#82248

Purpose

Gateway Donor vector containing IKBKE, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKBKE (IKBKE Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 T73I

Plasmid

#140939

Purpose

expresses PTPN11 T73I

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Tags

V5

Expression

Mammalian

Mutation

T73I

Promoter

EF1a

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPS2_p.R102Q

Plasmid

#81349

Purpose

Gateway Donor vector containing GPS2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GPS2 (GPS2 Human)

Use

Gateway entry vector

Mutation

R102Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

Plasmid

#172625

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr280Ala

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_TP53_p.C275F

Plasmid

#81399

Purpose

Gateway Donor vector containing TP53 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TP53 (TP53 Human)

Use

Gateway entry vector

Mutation

P72R; C275F; P278A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND1-Puro

Plasmid

#172643

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.L52R

Plasmid

#81604

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Mutation

L52R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_H2AFX_p.K134N

Plasmid

#81275

Purpose

Gateway Donor vector containing H2AFX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

H2AFX (H2AX Human)

Use

Gateway entry vector

Mutation

K134N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.D1045V

Plasmid

#81702

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CA (PIK3CA Human)

Use

Gateway entry vector

Mutation

D1045V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GLIPR1_WT

Plasmid

#81780

Purpose

Gateway Donor vector containing GLIPR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GLIPR1 (GLIPR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WIPI1_WT

Plasmid

#82055

Purpose

Gateway Donor vector containing WIPI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WIPI1 (WIPI1 Human)

Use

Gateway entry vector

Mutation

T31I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG3A_WT

Plasmid

#81826

Purpose

Gateway Donor vector containing REG3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG3A (REG3A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_XPO1_p.E571K

Plasmid

#81554

Purpose

Gateway Donor vector containing XPO1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XPO1 (XPO1 Human)

Use

Gateway entry vector

Mutation

E571K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSumo24P7vB4

Plasmid

#113073

Purpose

Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

Depositor

Bohdan Schneider

Article

Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

Insert

Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))

Tags

SUMO

Expression

Bacterial

Mutation

Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ACVR1B_WT

Plasmid

#81738

Purpose

Gateway Donor vector containing ACVR1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ACVR1B (ACVR1B Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSumo24P7

Plasmid

#113072

Purpose

Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

Depositor

Bohdan Schneider

Article

Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

Insert

Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))

Tags

SUMO

Expression

Bacterial

Mutation

Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7a (closed)

Plasmid

#160101

Purpose

Expresses murine Fbxw7 alpha in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_TCF7L2_WT

Plasmid

#81745

Purpose

Gateway Donor vector containing TCF7L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TCF7L2 (TCF7L2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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