We narrowed to 15,984 results for: grna

1. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

   Plasmid

   #188976

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g2 guide RNA
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGE010-Target2 (Mpphot)

   Plasmid

   #186729

   Purpose

   Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

   Depositor

   Yutaka Kodama

   Article

   Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

   Insert

   Mphot
   

   Expression

   Bacterial

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. shRNA-Ratio-PosCon

   Plasmid

   #183553

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Pbrm1 shRNA
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. shRNA-Ratio-NegCon

   Plasmid

   #183554

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Luciferase shRNA
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTAG4E

   Plasmid

   #176868

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_4E
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTAG5

   Plasmid

   #176869

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_5
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAG6E

   Plasmid

   #176870

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_6E
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTAG2

   Plasmid

   #176864

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_2
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAG3

   Plasmid

   #176866

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_3
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTAG4

    Plasmid

    #176867

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_4
    

    Use

    CRISPR

    Mutation

    WT

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sg-CMV51

    Plasmid

    #179915

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX552-rScn2a-3xV5-Syn-miRFP670

    Plasmid

    #182566

    Purpose

    For rat Nav1.2 knockin with 3xV5 at the extracellular loop

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    rat Scn2a gRNA and 3xV5 donor DNA
    miRFP670
    

    Use

    AAV and CRISPR

    Promoter

    Syn and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMCL8

    Plasmid

    #176184

    Purpose

    gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCL8_28bp

    Plasmid

    #176185

    Purpose

    plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCL9

    Plasmid

    #176186

    Purpose

    2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-TREX1 g3 Cas9-T2A-mCherry

    Plasmid

    #164252

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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