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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mApple

Plasmid

#96934

Purpose

Retroviral plasmid containing mApple for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Apple

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP12089

Plasmid

#222357

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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SRpHi3

Plasmid

#102543

Purpose

Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-sepHluorin

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2(ΔmScarlet)

Plasmid

#208185

Purpose

GFP positive, mScarlet-I negative control

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Insert

GFPmut2

Use

Reporter

Expression

Bacterial

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
ZO-1 shRNA 2968

Plasmid

#37208

Depositor

James Anderson, Alan Fanning

Article

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-1 shRNA (TJP1 canine)

Use

RNAi

Expression

Mammalian

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

Plasmid

#164263

Purpose

Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Type

Empty backbone

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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scFv-M-MLV-RT

Plasmid

#181809

Purpose

scFv-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV_RT

Use

Lentiviral

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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SRpHi1

Plasmid

#102541

Purpose

Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EYFP

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1002

Plasmid

#71820

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPyCas9

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pDB023f

Plasmid

#99217

Purpose

Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] T7RNAP(S12*,S203*)

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pIV101

Plasmid

#196671

Purpose

Enables recombination mediated gene delivery into Agrobacterium strains for subsequent transformation of plants

Depositor

Simona Radutoiu

Article

Ferguson et al PLoS One. 2023 Nov 1;18(11):e0291680. do

Type

Empty backbone

Use

Integration vector

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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petRNA-FANCF-v3'

Plasmid

#181803

Purpose

petRNA FANCF v3'

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

ribozyme-petRNA

Use

CRISPR

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MS2-pegRNA

Plasmid

#181811

Purpose

ms2 incorporated pegRNA-1.1

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MS2-pegRNA

Use

CRISPR

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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cp196V1.2

Plasmid

#52100

Purpose

A improved cpmCherry variant

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

cp196V1.2

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pCY 3080-01

Plasmid

#36226

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEmCitrine

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-kanMX

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19, containing H2-Kb c57/BL6 WK9

Plasmid

#102641

Purpose

Encodes sequence of the murine H2-Kb allele

Depositor

Sai Reddy

Article

Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

Insert

H2-Kd Balb/c

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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