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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

Plasmid

#187584

Purpose

Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SP100 (SP100 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-TFRC

Plasmid

#172420

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Fyn-Fyn Biosensor

Plasmid

#124655

Purpose

FRET biosensor. Fyn-tagged biosensor targeting to membrane

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRG705_lexO-AsiSI_LexA-TF_LEU2MX

Plasmid

#154817

Purpose

Inducible expression of AsiSI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AsiSI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-Venus

Plasmid

#153950

Purpose

piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B57

Plasmid

#135516

Purpose

Mammalian expression of myc-tagged HLA-B*57:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*57:1 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-DAP5-3XFLAG

Plasmid

#127330

Purpose

Express 3xFLAG tagged DAP5 protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T1 (+minintr, KDR)

Plasmid

#119200

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLX303-PCDH7-d

Plasmid

#108541

Purpose

Expresses human PCDH7 transcript variant d in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant d (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-fd [Chronos-GFP]

Plasmid

#84482

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [Chronos-tdTomato]

Plasmid

#84481

Purpose

AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PTK7DN-VSV

Plasmid

#65251

Purpose

Expression of VSV tagged dominant negative PTK7 in mammalian cells

Depositor

Axel Ullrich

Article

Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

Insert

PTK7DN (PTK7 Human)

Tags

VSV

Expression

Mammalian

Mutation

deleted AA 737-1071

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pHR KIF17 (1-738)

Plasmid

#60406

Purpose

Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

KIF17 (KIF17 Human)

Use

Lentiviral

Tags

GB1, Strep-Tag, and sfGFP

Expression

Mammalian

Mutation

Truncated version amino acids 1-738

Promoter

SFFV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-2xOsh2 PH domain [short]

Plasmid

#58829

Purpose

Expresses GFP-Tagged Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2xOsh2 PH domain [short] (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pFRT/TO/HIS/FLAG/HA-API5

Plasmid

#38074

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

API5 apoptosis inhibitor 5 (API5 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I beta

Plasmid

#22155

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 FEE-AAA

Plasmid

#21942

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, FEE-AAA

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only