We narrowed to 13,037 results for: Mpl

1. pFastBac-ATG101-ATG13 HORMA

   Plasmid

   #99332

   Purpose

   Mammalian expression of one subcomplex from the Human ULK1 complex

   Depositor

   James Hurley

   Article

   Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

   Inserts

   ATG101 (ATG101 Human)
   ATG13 (ATG13 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   p10 and pH

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1.1-mCherry

   Plasmid

   #162772

   Purpose

   Fluorescent reporter for CHO expression studies

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Insert

   red fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1.2-GS-eGFP

   Plasmid

   #162771

   Purpose

   Fluorescent reporter for CHO expression studies

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. COVID-SARS2 NSP14/10

   Plasmid

   #159613

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

   Depositor

   Opher Gileadi

   Article

   Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

   Inserts

   Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
   Covid-SARS2 Nsp10
   

   Use

   Tags

   His6, TEV cleavage site and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP(1-158)-beta-1 in pcDNAI/Amp

   Plasmid

   #54468

   Purpose

   An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
   

   Use

   Tags

   YFP(1-158) was fused to the amino terminus of Gbe…

   Expression

   Mammalian

   Mutation

   GBeta-1 was amplified via PCR, which removed an i…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

   Plasmid

   #183080

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Tags

   Expression

   Mutation

   -124C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

   Plasmid

   #183081

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Tags

   Expression

   Mutation

   -146C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEH_AAV_TERT_prom_C7C_1.8kb

   Plasmid

   #183079

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Tags

   Expression

   Mutation

   Wildtype promoter, Exon 1 C7C (C21>T21) silent…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcGFP-IVT

   Plasmid

   #205145

   Purpose

   IVT template of AcGFP with MCS in frame and polyA

   Depositor

   Igor Ulitsky

   Insert

   AcGFP1
   

   Use

   In-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDW139

    Plasmid

    #227336

    Purpose

    Template for in vitro Pol II transcription

    Depositor

    Michelle Wang

    Insert

    Gap DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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