Addgene

Showing: 1061 - 1080 of 1099 results

Results per page:

Sort results by:

pN1-RnKIF5C(1-560)-Halo-Flag

Plasmid

#188071

Purpose

Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

Depositor

Kristen Verhey

Article

Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

Insert

rat KIF5C (Kif5c Rat)

Use

Tags

Flag and Halo

Expression

Mammalian

Mutation

aa1-560

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
eDrHER2

Plasmid

#183995

Purpose

Optically controlled eDrHER2 expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrHER2 (ERBB2 Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
eDrFGFR1

Plasmid

#183994

Purpose

Optically controlled eDrFGFR1 expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrFGFR1 (FGFR1 Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC2T-actin

Plasmid

#108968

Purpose

expresses BC2-tag-actin in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

beta-actin (ACTB Human)

Use

Tags

BC2-tag

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1 VAMP2 R125TAG

Plasmid

#69877

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2 (Vamp2 Rat)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Amber and Ochre stop codons in the former linker …

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d344-873, K45E

Plasmid

#21735

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Promoter

Available since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
macroH2A1.1-GFP (pc2188)

Plasmid

#223707

Purpose

Expression of macroH2A1.1 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.1-GFP (Macroh2a1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
macroH2A1.2-GFP (pc2189)

Plasmid

#223708

Purpose

Expression of macroH2A1.2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.2-GFP (Macroh2a1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
vPyACR_2164382

Plasmid

#153029

Purpose

Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

vPyACR_2164382

Use

Tags

eYFP

Expression

Mammalian

Mutation

vPyACR_2164382 was C-terminally truncated to incl…

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
vPyACR_21821

Plasmid

#153030

Purpose

Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

vPyACR_21821

Use

Tags

eYFP

Expression

Mammalian

Mutation

vPyACR_21821 was C-terminally truncated to includ…

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LAMP-mCherry-CRY2

Plasmid

#102249

Purpose

Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

LAMP-mCherry-CRY2 (LAMP1, CRY2 Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EB3-mNeonGreen

Plasmid

#191761

Purpose

Expression of mNeonGreen fused to EB3

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

EB3-mNeonGreen (MAPRE3 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PEX-mCherry-CRY2

Plasmid

#102250

Purpose

Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

PEX-mCherry-CRY2 (PEX3, CRY2 Human, Mustard Weed)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-beta2(616-951)-GFP Double

Plasmid

#100729

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform, deletion of clathrin box motif and …

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-beta2(616-951)-GFP ∆CBM

Plasmid

#100728

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform, deletion of clathrin box motif

Promoter

CMV

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-beta2(616-951)-GFP Y215A

Plasmid

#100727

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform Y215A mutation (Y815A in full length)

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP2-Actin-C-18

Plasmid

#178965

Purpose

Expresses the fusion protein of miRFP2-Actin-C in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2-actin

Use

Tags

actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge