We narrowed to 6,731 results for: tac

1. pGEX6P1‐Afu‐rnhBΔPIP

   Plasmid

   #108696

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C-terminal truncation (7aa) removing PIP box

   Promoter

   tac

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-SF3B2 wild type aa 401-550 fragment

   Plasmid

   #67615

   Purpose

   bacterial expression of wild type GST-SF3B2 fragment (401-550)

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   splicing factor 3b subunit 2 (SF3B2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   fragment of amino acids 401-550

   Promoter

   tac

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Intersectin I SH3 E domain (human)

   Plasmid

   #47417

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   ITSN1 intersectin 1 (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain E

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. RBMS3_2xRRM_pGEX

   Plasmid

   #135142

   Purpose

   Recombinant expression of RBMS3 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBMS3 (RBMS3 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. RBM24_RRM_pGEX

   Plasmid

   #135148

   Purpose

   Recombinant expression of RBM24 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBM24 (RBM24 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST-SF3B2 R508K aa 401-550 fragment

   Plasmid

   #79674

   Purpose

   bacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   splicing factor 3b subunit 2 (SF3B2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 451-550 with R508K

   Promoter

   tac

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-4T-1 dSesn (1187)

   Plasmid

   #66919

   Purpose

   bacterial expression of dSesn

   Depositor

   Michael Karin, Jun Hee Lee

   Article

   Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

   Insert

   Sesn (Sesn Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX6P1‐Afu rnhB

   Plasmid

   #108694

   Purpose

   For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Insert

   rnhB (AF_RS03165 Archaeoglobus fulgidus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GST-PC-2-CTS

   Plasmid

   #86068

   Purpose

   A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Polycystin-2 (Pkd2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 1-15

   Promoter

   tac

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T-1_OCT4_T234E_S235E

    Plasmid

    #40635

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    T234E_S235E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGEX4T-1_OCT4_T234E

    Plasmid

    #40636

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    T234E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGEX4T-1_OCT4_T234A

    Plasmid

    #40637

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV cleavage, and Thrombin cleavage

    Expression

    Bacterial

    Mutation

    T234A

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGEX4T-1_OCT4_S235E

    Plasmid

    #40638

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    S235E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pGEX4T-1_OCT4_S235A

    Plasmid

    #40639

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

    Expression

    Bacterial

    Mutation

    S235A

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. polySUMO1

    Plasmid

    #221066

    Purpose

    Expression of polySUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL-MBP-Cdc42-T17N

    Plasmid

    #214289

    Purpose

    Express dominant negative Cdc42 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative Cdc42 (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Cdc42-T17N

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMAL-MBP-FRB-Cdc42/N12

    Plasmid

    #214290

    Purpose

    Express split-Cdc42 fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Cdc42 fragment fused with FRB (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMAL-MBP-Rac1-Q61L

    Plasmid

    #214292

    Purpose

    Express constitutively active Rac1 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active Rac1 (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Rac1-Q61L

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMAL-MBP-Rac1-T17N

    Plasmid

    #214293

    Purpose

    Express dominant negative Rac1 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative Rac1 (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Rac1-T17N

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL-MBP-FRB-Rac1/N12

    Plasmid

    #214294

    Purpose

    Express split-Rac1 fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Rac1 fragment fused with FRB (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge